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Nucleic Acids and Oligonucleotides

The modification and conjugation of nucleic acids and oligonucleotides is quite different from other biopolymers, as they are rather inert to common bioconjugation reagents and conditions. Although there are reagents that can modify the base units, base modification may affect the binding capacity or propensity of nucleic acids and oligonucleotides. Many biopolymers cannot withstand the final basic cleavage conditions of solid phase oligo synthesis, so oligo bioconjugation usually is done in solution after oligo synthesis. On the other hand, small molecules may be incorporated into the oligo during oligo synthesis. Oligos are generally modified first with extra functional groups suitable for bioconjugation at the 5’ or 3’ end during oligo synthesis. Some popular 5’ or 3’ modifications for oligos are amine, thiol, and phosphate. Oligonucleotides can also contain modified bases and biomolecules conjugated at the modified base. CellMosaic offers versatile oligonucleotide labeling and conjugation strategies, depending on the application of the product.

At CellMosaic, we carry Personalized Conjugation Kits (PerKit™) for oligo labeling and conjugation, please click here to check our PerKit™ oligo labeling and conjugation kits.

For large scale or project beyond the scope of PerKit™ configuration, please contact us for a quote.

Figure 1. HPLC analysis of an oligo-peptide conjugate synthesized at CellMosaic through a thioether bond.


 Selected Examples: 

  • Depiction of AP-Oligo Conjugate Example 1: Preparation of single labeled AP-oligo conjugates.

    AP—Oligo Conjugate

    Alkaline phosphatase (AP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, such as nucleotides and proteins. It is used to detect target proteins through chromogenic, chemiluminescent, or fluorescent outputs...

  • Depiction of Labeled Oligo (single stranded) Scheme: oligo labeling through an NHS ester

    Oligonucleotide Labeling

    Oligo nucleotides (DNA or RNA) can be labeled anywhere (3', 5', or via a modified base). The conjugation can be performed in either solid phase or post-synthesis solution phase. Usually a single pure labeled oligo can be obtained. The advantages of...

  • Example structure of peptide-oligo conjugate Example 1: Synthesis and purification of BHQ2 oligo-peptide conjugates.

    Peptide—Oligo Conjugate

    At CellMosaic®, we routinely synthesize oligo-peptide conjugates for customers, in particularly, the ones that customers have difficulties in obtaining themselves due to the properties of the starting oligos or peptides. We use various strategies to...