Biopolymer Modification
For many bioconjugation applications, it is necessary to introduce functional groups or specialized linkers onto a biopolymer before labeling or conjugation. The figure on the left illustrates a typical biopolymer modification process, in which a biopolymer (M1) reacts with a modification reagent (M2) to introduce a desired functional group (FG).
Over the years, CellMosaic has developed a broad portfolio of technologies for introducing unique functional groups onto biopolymers, including aldehyde, ketone, maleimide, DBCO, azide, and thiol groups. These functional groups enable highly selective downstream labeling and conjugation reactions. For example, introducing an aldehyde group allows the biopolymer to undergo site-selective conjugation through reductive amination, oxime ligation, or hydrazone formation.
We routinely modify a wide range of biopolymers, including antibodies, antibody fragments, enzymes, proteins, peptides, peptidomimetics, DNA, and nucleic acid analogs. For more information on functional group introduction, please click here.
CellMosaic also offers antibody engineering services to prepare Fab, Fab', F(ab')2, and bispecific antibody fragments using proprietary combinations of chemical and enzymatic methods.