Shop by Category

ADC Kits

ADC Personalized Conjugation Kits (PerKits™)

Download Documents
ADC FLYER
APPLICATION NOTE

CellMosaic ADC PerKits™ are available to prepare antibody conjugates through surface amines or reduced thiols. Some ADC kits apply to general payloads with certain functional groups. Some ADC kits supply the selected, standard linkage molecules conjugated to a selected payload, ready with the appropriate reactive modifications to directly attach the payload and linker to your specific antibody of interest. The kits are complete with all reagents and procedures for attaching the selected payload directly to your antibody and purifying the final ADC. All you need to supply is your antibody and standard lab equipment.

A typical CellMosaic personalized ADC kit results in the preparation of approximately 2-3 mg of final ADC in buffer with an average DAR of 2-4. Purities are typically >90% conjugate, free of or with less than 5% of unreacted payload. Individual results can vary depending on the specific antibody system you are using and the hydrophobicity of your payload. Kit instructions are complete, easy to follow, and can typically be carried out within 6 hours (with less than 1-hour hands-on time).

AqT™ ADC Evaluation

In the event that a drug conjugate cannot be made using classical linkers or the effectiveness of the drug conjugates are compromised by the number of toxins loaded onto the antibody using CellMosaic PerKits™ ADC, CellMosaic has developed super-hydrophilic and water soluble AqT™ linker for this purpose. AqT™ADC Evaluation Kits are currently available to perform conjugations at CellMosaic and evaluate the benefit of AqT™ linkers for ADC on a small scale. Click here for more information.

Other products on the market for antibody screening may utilize a general secondary antibody, or a general antibody affinity coupling (such as protein-A), to provide indirect conjugation, but these indirect conjugation approaches for ADCs can significantly impact the hydrodynamic size and physiological behaviors (for example, changing cleavage and degradation mechanisms), so these products do not provide a fully representative approach for evaluating your novel ADC in screening or for proof-of-concept (POC) studies.

 

Perkit™ ADC Selection Guide

 Product#
 Protein
 Drug
 Scale of  Reaction (Standard Kit)
 Labeling Chemistry
 Linkage
 CM11429 IgG Ab No drug (VC-PAB linker only) 1-3 mg Reduced thiol Releasable
 CM51403  IgG Ab

 Acid

 1-3 mg  Surface amine  Stable 
 CM11410 (x1, x3)  IgG Ab  DM1  0.1 mg, 1-3 mg  Surface amine  Stable 
 CM11406 (x1, x3)  IgG Ab  Doxorubicin  1-3 mg  Surface amine  Stable 
 CM11407 (x1, x3)  IgG Ab  Methotrexate  1-3 mg  Surface amine  Stable 
 CM11409 (x1, x3)  IgG Ab  MMAE  0.1 mg, 1-3 mg, 30 mg  Reduced thiol  Releasable 
 CM11422 (x1, x3)  IgG Ab  MMAF  1-3 mg  Reduced thiol  Stable 
 CM11425 (x1, x3)  IgG Ab  MMAF  1-3 mg  Reduced thiol  Releasable 
 CM11408 (x1, x3)  IgG Ab  SN38  1-3 mg, 30 mg  Surface amine  Releasable 
 CM11431 (x1, x3)  IgG Ab Deruxtecan  1-3 mg  Reduced thiol  Releasable 
 CM11416 (x1, x3)  F(ab')2  MMAE  0.73-2.2 mg  Reduced thiol  Releasable 

 CM11419 (x1, x3)

 F(ab')2  DM1  0.73-2.2 mg  Surface amine  Stable
(Note: additional kits configurations can be added depending on the request from customers. Please inquire if you do not see your current configuration of interest listed or request a custom bioconjugation service)

 


Selected Citations from Customers who Use ADC Prepared from CellMosaic's ADC Kits 

1. Lofgren, Kristopher A.; Sreekumar, Sreeja; Jenkins, Charles E., Jr.; Ernzen, Kyle J.; Kenny, Paraic A. "Anti-tumor efficacy of an MMAE-conjugated antibody targeting cell surface TACE/ADAM17-cleaved Amphiregulin in breast cancer." Antibody Therapeutics 2021, 4 (4), 252-261. 

2. Neetha Parameswaran, Liping Luo, Lingjun Zhang, Joel Chen, Frank P. DiFilippo, Charlie Androjna, David A. Fox, Sarah L. Ondrejka, Eric D. Hsi, Deepa Jagadeesh, Daniel J. Lindner & Feng Lin. "CD6-targeted antibody-drug conjugate as a new therapeutic agent for T cell lymphoma" Leukemia 2023 Aug 12. doi: 10.1038/s41375-023-01997-8. Online ahead of print. 

3. Micalizzi, Douglas S.; Che, Dante: Nicholson, Benjamin T.; Edd, Jon F.; Desai, Niyati; Lang, Evan R.; Toner, Mehmet; Maheswaran, Shyamala; Ting, David T.; Haber, Daniel A. "Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody." PNAS 2022, 43 (119), e2209563119.