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Biopurification Services
CellMosaic offers purification solutions spanning small molecules to complex biopolymers, with a focus on the isolation of discrete, structurally defined bioconjugates. Our approach emphasizes method development based on the physicochemical and structural properties of each conjugate to achieve high purity and batch consistency.
Peptide and oligonucleotide conjugates are typically purified via reversed-phase HPLC, leveraging hydrophobic interactions for high-resolution separation. Antibody-based conjugates are commonly purified using affinity chromatography (e.g., Protein A/G), ensuring specificity and preservation of biological activity. Other macromolecular conjugates, such as HRP-based systems, are often purified using size-exclusion chromatography to separate species based on hydrodynamic radius.
Custom purification strategies can be developed for complex or non-standard conjugates. Please request a quote to discuss your specific project.
Standard Services
| Code | Service Type | Description |
|---|---|---|
| PS0001 |
Standard HPLC
|
Standard HPLC purification uses a C18 column for the purification of peptides (small proteins) and oligos. Purity > 90%.
|
| PS0002 |
Gel filtration
|
Purification of conjugates based on the molecular weight (size-exclusion chromatography).
|
| PS0003 |
Protein A chromatography
|
Purification of IgG molecules.
|
| PS0004 |
Antigen affinity chromatography
|
For specific antibody purification. Please supply immobilized antigen media or request a custom immobilization.
|
| PS0005 |
Avidin/streptavidin purification
|
Use immobilized iminobiotin column
|
| PS0006 |
Metal ion affinity chromatography
|
For purification of proteins containing a His tag or purification of antibody-enzyme conjugate.
|
HPLC: high performance liquid chromatography.