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HPLC Analysis

At CellMosaic, we have tremendous experience in analyzing and purifying a variety of small organic compounds, biopolymers, and bioconjugates by high pressure liquid chromatography (HPLC) utilizing different media and columns. We established methods for analyzing peptides, oligos, proteins, antibodies, and their conjugates. For the development of HPLC methods involving special molecules, we encourage you to talk with our specialist.

  • Reverse-phase HPLC analysis: This type of analysis involves HPLC/UV analysis of small organic compounds, peptides, oligos, antibodies, enzymes, proteins, and their conjugates. We help customers develop HPLC methods for analyzing bioconjugates, such as proteins, peptides, oligos, and ADCs, among others. We have a variety of reversed phase columns, including C4, C8, C18, and phenyl-modified columns, with which to perform method development. Generally, we can narrow down the type of column and buffers needed for separation based on the nature of the analyte.
  • Size-exclusion chromatography: This chromatographic method is suitable for analyzing mixtures of biopolymers, separating the components according to size. It can be used to determine the approximate MW of the molecules. In the case of bioconjugate analysis, it can be used to assess the amount of unlabeled molecules and degree of bioconjugate aggregation in a sample.
  • Hydrophobic interaction chromatography (HIC): This chromatographic method is good for analyzing proteins or antibody/protein bioconjugates based on their hydrophobicity. The theory behind HIC is similar to reverse-phase chromatography; however, the degree of substitution of HIC adsorbents is diluted compared to reverse-phase chromatography. Thus, the compound can be eluted in a more polar and less denaturing environment. For example, HIC has been used extensively to analyze drug loading in ADCs or protein-drug conjugates. 
  • Anion and cation exchange chromatography: This type of chromatography uses the charged state of the molecule to achieve separation. It is generally a mild and selective method for analyzing peptides, oligos, or proteins based on the charge. 

Pricing Information for Some Standard HPLC Services