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    • NeIon™ Technologies

    NeIon™ Products

    NeIon™ technologies utilize specifically designed organo-trifluoroborate compounds to label analytes of interest, enhancing their detection sensitivities in negative ion mode during Mass Spectrometry (MS) analysis. NeIon™ technologies also enable the quantification of trace amount of these analytes.

    Organo-trifluoroborates are stable, negatively charged molecules that serve as effective MS tags due to their properties.

    • Permanent negative charge (pH independent) unlike –PO3−, –SO3−, –COO− groups, leading to enhanced sensitivity.
    • Produce (or induce) predictable fragmentation in MS/MS mode, offering improved selectivity.

    Example of a NeIon™ tag.

    neion-example.png

     

    Why Negative Ion Mode 

    Negative Mode Analysis

    • Very few MS analyses are conducted in negative ion mode because only a limited number of molecules are naturally negatively charged.
    • Selectively labeling analytes with a negative ion MS tag can dramatically enhance sensitivity (100 to 1000-fold) by improving the signal-to-noise ratio.
    • To the best of our knowledge, no MS tag specifically designed to enhance sensitivity/selectivity for negative ion mode analysis has been reported.

    Positive Ion Mode Analysis

    • The majority of MS analyses are performed in positive ion mode because most molecules (both small and large) ionize easily in this mode.
    • However, this leads to a high probability of interference and background noise.
    • Sensitivity-enhancing mass tags have been developed for positive ion mode, but intellectual property landscape is crowed. 

    positive-vs-negative-ion.png

    Fragmentation pattern of NeIon

     

    fragmentation.png

     Multiplexing with NeIon™ Tag

     

     multiplexing.png

     

     

    Patent and License Restrictions

    NeIon™ technologies are covered under US patent numbers 9346833B2 and 9518067B2 in the name of CellMosaic, Inc. The purchase of NeIon™ products includes a limited license for internal research and development use only, not for any commercial purposes. Commercial use includes, but is not limited to:

    1. The sale, lease, license or other transfer of the material or any material derived or produced from it.

    2. The sale, lease, license or other grant of rights to use this material or any material derived or produced from it.

    3. The use of this material to perform services for a fee for third parties, including contract research and drug screening.

    If you are interested in using NeIon™ reagents for commercial purposes, please contact us for more information.