Antibody UM171 Conjugation Kit

Product Description

CellMosaic® has designed this kit to label any antibody (IgG type) with UM171 through a stable amide bond. The user provides the antibody, while the kit includes UM171 N-Hydroxysuccinimide (NHS) ester.  This UM171-NHS ester couples directly to the antibody's surface amines in a single step. The final product is then purified to remove any unreacted UM171.

UM171 is a small molecule first discovered in Canada and published in 2014. It is known for its ability to enhance hemapoetic stem cell renewal (Fares I. et. al. Science 2014, 345, 1509-1512). UM171 activates the CULLIN3^KBTBD4 ubiquitin ligase, which specifically targets the LSD1-CoREST repressor complex for proteasomal degradation (Chagraoui J. et al. Cell Stem Cell. 2021, 28, 48-62). A phase 1-2 trial of UM171-expanded cord blood (CB) transplants demonstrated safety andd favorable preliminary efficacy.

This kit provides materials to conjugate 1 to 3 mg of one (1) antibody sample (CM11433x1) or three (3) antibody samples (CM11433x3) of IgG with UM171 via a stable amide bond. 

Drug Information

• Name: UM171
• Synonym: 4-N-[2-benzyl-7-(2-methyltetrazol-5-yl)-9H-pyrimidio[4,5-b]indol-4-yl]cyclohexane-1,4-diamine, trans-N1-(7-(2-Methyl-2H-tetrazol-5-yl)-2-(phenylmethyl)-9H-pyrimido(4,5-b)indol-4-yl-cyclohexanediamine.
• CAS number: 1448724-09-1
• Mechanism of action (list only few): Activates the CULLIN3^KBTBD4 ubiquitin ligase, which specifically targets the LSD1-CoREST repressor complex for proteasomal degradation.
• Medical usage: Enhance hematopoietic stem cell renewal.

Requirement for Antibody

1. Preferably >90% pure by gel electrophoresis
2. Total amount: 1-3 mg of protein content as measured by UV. Note: The accuracy of your protein measurement is the single most important factor in obtaining an optimized DAR of 4. The user manual contains instructions for measuring protein amount under the "Other Considerations" section.

Key Features of this PerKit™

• Provides a simple and efficient method to label IgG with UM171
• Ensures a stable linkage 
• Quick and easy preparation: 4 h process with less than 1 hour of hands-on time
• Includes all necessary reagents and supplies for both preparation and purification
• Optimized Drug-to-Antibody Ratio (DAR): Average 3-5 UM171 molecules per antibody
• Features a stabilizing buffer for long-term storage.
• Delivers >99% of conjugated products, free of unreacted UM171
• Options to choose tailored services at CellMosaic® after conjugation

After conjugation: you can choose to send your sample to CellMosaic® for HPLC analysis and determination of the DAR.

Conjugation Chemistry

Protocol

Schematic diagram of the workflow for preparing antibody-UM171 Conjugates.

Typical Kit Performance Data (LC Analysis, CellMosaic)

Antibody information: A therapeutic antibody (human IgG1 subtype)

Kit Lot number: S538.S7.1203D and S538.S8.1203D

Figure 1: SEC HPLC Analysis of purified UM171-ADC following procedure of DCM11433, using the full amount of UM171 NHS ester (Inset: UV/Vis spectra of UM171-ADC). Scale of the reaction: 1 mg of Antibody.

Summary of the result:

Related Products

Hydrophobic Interaction Chromatography (HIC) Buffer Set (Product#: CM02025): For ADCs prepared via surface amines of the antibody, hydrophobic interaction chromatography (HIC) HPLC can be used to check if an antibody is labeled or not. However, due to the highly heterogeneous nature of surface amine labeling, antibody loaded with the same number of drugs (same DAR) may have slightly different hydrophobicity. For a typical DM1 ADC, a broad peak will be seen without clear separation of the peaks. CellMosaic® offers HIC buffer for our customers to use with any HIC column. The CM02025 product sheet contains all of the information and methodology needed to run an HIC HPLC analysis. If you do not have access to an HPLC facility, you can send your sample to CellMosaic for analysis.

Size Exclusion Chromatography (SEC) HPLC Protein Standards (Product #: CM92004 and CM92005): DM1 is very hydrophobic. This kit is designed to minimize the aggregation and precipitation issues generally seen with DM1 labeling. However, you may still notice some solid precipitate out or ADC aggregation during the reaction. The precipitate will be removed during purification. Depending on the properties of your antibody, recovery will be 30-60%. If you are concerned with the aggregation, you can use size exclusion chromatography (SEC) to check the extent of aggregation. SEC separates the conjugates by apparent molecular weight (MW) or size in aqueous solution. The larger the MW of the conjugate, the earlier it elutes. By comparing the SEC profile of unlabeled IgG and the ADC, you can estimate how much aggregation is in the ADC. CellMosaic® offers two SEC standards for our customers to use with any SEC column. The CM92004 product sheet contains all of the information and methodology you need to run an SEC HPLC analysis. If you do not have access to an HPLC facility, you can send your sample to CellMosaic® for analysis.

ADC stabilizing PBS buffer (5x) (Product #: CM02022): CM02022 contains 5x PBS buffer and other stabilizers to prevent the hydrophobic drugs from interacting with each other during storage, which would cause the ADCs to precipitate out. Stabilization buffer also helps preserve the structure of the ADCs during lyophilization. The buffer is biocompatible and can be used directly for any in vitro and in vivo studies