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Bioassay Services

CellMosaic offers bioassay services that support the characterization and quality assessment of the bioconjugates we manufacture in-house. These assays are often integrated into our bioconjugation services. Typical bioassays include:

  • Functional Group Assays
    A key aspect of bioconjugation is understanding the characteristics of the biopolymers involved and quantifying their reactive functional groups. For example, when cysteine (Cys) residues are used in a bioconjugation reaction, it is important to determine the amount of reactive Cys present because free thiols are readily oxidized upon exposure to air. Similarly, when a drug is conjugated to a protein through surface amine groups, measuring the free amine content before and after conjugation can be used to estimate drug loading.
  • Biopolymer Activity Assays
    Activity assays for biopolymers, including enzymatic activity assays for horseradish peroxidase (HRP) and alkaline phosphatase (AP), as well as biotin assays for biotinylated biopolymers.
  • Fluorescence Assays
    Fluorescence measurements for biopolymers labeled with fluorescent dyes.
  • Endotoxin Assays
    Measurement of endotoxin levels in bioconjugates.

Please contact us if you have specific bioassay requirements or would like to discuss a customized testing plan.

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