Product Number CM5xxxx
CellMosaic’s product number (catalog number or SKU) CM5xxxx is generally assigned to antibody, enzyme and other protein related reagents and kits.
CM51100: ADC Control hIgG1-MMAE
Do you have any references concerning this product, especially the concentration to use. What would be an optimal concentration to use as a control?
We don’t have any references for this product because it is a new product made by us. As a general rule, for any control usage, you should use the same drug concentration as your binding ADC (after taking into consideration the DAR). The concentration of binding ADC you use will depend on your application. For cell viability studies to determine the IC50, you can use various concentrations ranging from 0.001 µg/mL to 10 µg/mL.
CM52418: Ova-Peptide Conjugation Kit
My peptide sequence has two cystine residues. Will I be able to use the Ova-Peptide Conjugation Kit?
With two active cysteines you may run into the situation where your peptide may crosslink two ova together. However, as this kit is designed to produce relatively high loading peptide-ova conjugates, the dimerization and polymerization will be relatively difficult to form because the priority will be to form the Ova-Peptide conjugate and use up all of the active maleimide groups.
For modifications to the protocol to help with specific features of your peptide we offer a PerKit sheet, where we write a modified protocol for you.
CM53405: Perkit™ Antibody-Oligo Conjugation Kit
Is it possible to conjugate double-stranded DNA in which only one strand is aminated?
Yes, you will be able to use this conjugation kit for double-stranded DNA. For dsDNA >30 bp we recommend using the configuration for labeling long oligo: >60 bases (CM53405.3x1 for 1 reaction and CM53405.3x3 for 3 reaction). Depending on the melting temperature of your dsDNA, some single-stranded DNA may be present in the reaction mixture during step 1A (B6) but it should anneal back during purification (steps E1-E7).
Is it possible to conjugate double-stranded DNA in which only one strand is aminated?
Yes, you will be able to use this conjugation kit for double-stranded DNA. For dsDNA >30 bp, we recommend using the configuration for labeling long oligo: >60 bases (CM53405.3x1 for 1 reaction and CM53405.3x3 for 3 reaction). Depending on the melting temperature of your dsDNA, some single-stranded DNA may be present in the reaction mixture during step 1A (B6), but it should anneal back during purification (steps E1-E7).
Did you internally test the conjugation efficiency for different hosts and isotypes?
We use hIgG1 for testing the antibody-oligo conjugation efficiency of our kit. As the labeling site on the antibody is at the surface amines, we do not expect too much variation in the conjugation efficiency for isotypes or antibodies produced from different hosts. We have performed some custom antibody-oligo synthesis with other isotypes of the antibodies for customers previously and have not seen too much of a difference with this chemistry. However, you may see some variation if your oligo is not pure enough or the oligo is too long and hydrophobic.
What is the salt concentration of your conjugating buffer (Buffer B)? Our antibody is very sensitive to salt concentration.
Unfortunately, we are not able provide the exact salt concentration other than to mention that the salt concentration in Buffer B is
Does this kit generate stable or releasable conjugates? What is the expected method for payload release?
The CM53405 kit will generate antibody-oligo conjugate via a stable thiol ether bond. Once inside the cell, the antibody can be digested by protease enzymes, and the oligo is released with one amino acid at the end. If you need a release linker, such as VC-PAB, we can make it via our custom conjugation service. We have not yet developed a kit for such a configuration (Answered on Jan 16, 2023).
CM52420: Streptavidin Antibody Conjugation Kit
Would the streptavidin-antibody conjugate I make with this kit work as a bridging molecule between biotinylated proteins and protein AG beads?
Yes, this could be used to bridge between protein and antibody via streptavidin-biotin labeling. There may be slightly weak protein AG binding with the antibody if the binding sites get blocked or hindered by the streptavidin.