SepSphere™ Oligo Immobilization Kit via Carboxylic Acid

Product Description

CellMosaic® has designed this oligo immobilization kit to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. The kit provides agarose beads derivatized with carboxylic acid functional groups (5-20 μmol COOH per mL of resin). Using the kit components, customers immobilize their amino containing oligo onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.

Key Features of this Immobilization Kit:

• Minimum requirement for immobilization set-up
• All reagents, buffers, and plasticwares are included 
• Modestly activated carboxylic acid agarose
• One step immobilization with very mild reaction conditions
• Easy preparation with air push mechanism for washing: less than 1 hr hands-on time 
• Stable linkage 

Specification of the Solid Support

• Functional groups: carboxylic acid (COOH)
• Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
• Particle size: 45-165 um 
• Mean bead size: 90 um   
• Ligand concentration: 5-20 μmol COOH per mL agarose

 Specification of the Immobilized Product

• Immobilization chemistry: immobilized via stable amide bond
• Chemical stability: stable 
• pH Stability: stable     

 Requirement for oligo 

• Preferable 5' C6 or 3' C6 amino modified oligo
• HPLC purified and lyophilized
• Total amount: 0.1-2 μmol 
• Additional components of acid or other molecules containing primary or secondary amine groups and thiol should NOT be present
• Potential interfering compounds for labeling and conjugation reactions:
  1. Thiols: e.g., DTT and mercaptoethanol
  2. Primary and secondary amines: e.g., ammonium acetate, ammonium bicarbonate, ammonium tartrate, ammonium citrate, ammonium phosphate, AMPD [2-amino-2-methyl-1,3 propanediol], aminoguanidine bicarbonate salt, ethanol amine, Gly-gly, Tris buffer, piperidine buffers.