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  • About Us
    • Our History
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    • Our Approach
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  • Products and Services
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    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
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    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
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    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
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  • Antibody-Drug Conjugate (ADC) Kits and Solution
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    • Routine ADC Synthesis
    • ADC Controls
    • Buffers and others
  • Drug Conjugation Kits and Solutions
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    • Routine ADC Synthesis
    • ADC Controls
    • Buffers and others
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    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
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    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Mass Tags
    • Modified Biomolecules and Conjugates
    • Others
  • Routine Bioconjugation
    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
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    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
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  • SepSphere™ Oligo Immobilization Kit for Phosphate DNA
  • SepSphere™ Oligo Immobilization Kit for Phosphate DNA

    SepSphere™ Oligo Immobilization Kit for Phosphate DNA

    SepSphere™

    MSRP:
    Now: $752.00
    (You save )
    SKU:
    CM71403
    Availability:
    MTO (make to order) kit, usually ships within 2-3 week.
    Shipping:
    Calculated at Checkout

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    Product Description

     

    Download Documents (Coming Soon)
    SDS USER MANUAL

    CellMosaic® has designed this oligo immobilization kit to work with oligo containing a 5' phosphate group. For PCR product you can order a primer with a 5'-phosphate group or use polynucleotide kinase to put the phosphate at the end. You can also order phosphate containing oligo directly from many commercial oligo suppliers. The kit provides agarose beads derivatized with carboxylic acid functional groups (5-20 μmol COOH per mL of resin). Using the kit components, the customer immobilize the phosphate containing oligo onto SepSphere™ agarose beads in two steps following CellMosaic® standard protocol.

    Scale of the reaction: 5 mL


    Key Features of this Immobilization Kit:

    • Minimum requirement for immobilization set-up
    • All reagents, buffers, and plasticwares are included 
    • Modestly activated carboxylic acid agarose
    • One step immobilization with very mild reaction conditions
    • Easy preparation with air push mechanism for washing: less than 1 h hands-on time 
    • Stable linkage

    Specification of the Solid Support

    • Functional groups: COOH
    • Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
    • Particle size: 45-165 um 
    • Mean bead size: 90 um   
    • Ligand concentration: 5-20 μmol COOH per mL agarose

     Specification of the Immobilized Product

    • Immobilized chemistry: immobilized via stable amide bond
    • Chemical stability: stable 
    • pH Stability: stable     

     Requirement for oligo 

    • >90% by gel electrophoresis 
    • Total amount: 0.1-2 μmol 
    • >10 nucleotide bases

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