Product Description
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CellMosaic® has designed this personalized peptide immobilization kit to work with thiol containing peptide. If your peptide does not have a free thiol for immobilization, you can order a peptide with a Cys placed either at C-termini or N-termini. This kit provides agarose beads derivatized with maleimide functional groups (5-20 μmol maleimide per mL of resin). Using the kit components, customers can immobilize their thiol containing peptide onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.
Key Features of this Immobilization Kit:
- Minimum requirement for immobilization set-up
- All reagents, buffers, and plasticwares are included
- Modestly activated maleimide agarose (5-20 μmol maleimide per mL of resin)
- One step immobilization with very mild reaction conditions
- Easy preparation with air push mechanism for washing: less than 1 hr hands-on time
- Stable linkage (no leakage of the peptides from the resin under acid or base condition)
- Options to choose tailored custom services at CellMosaic® prior to and after conjugation in your lab:
Prior to conjugation, you can supply your peptide information when you place your order and CellMosaic® will give recommendations for the conjugation if your peptide has special features (complementary service);
After conjugation you can choose to send your sample to CellMosaic® for HPLC analysis of the peptide samples for determination of the peptide loading.
Specification of the Solid Support
- Functional groups: maleimide
- Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
- Particle size: 45-165 μm
- Mean bead size: 90 μm
- Ligand concentration: 5-15 μmol maleimide per mL agarose
Specification of the Immobilized Product
- Immobilization chemistry: immobilized via thiol ether bond
- Chemical stability: stable
- pH Stability: stable
Requirements for peptide
- Cys containing peptide. If your peptide does not have a Cys, add a Cys at either C-terminus or N-terminus
- HPLC purified and lyophilized, stored at ≤-20°C as lyophilized solid
- Preferably >90% pure by C18 HPLC
- Total amount: 1-20 mg
- Does not contain any other reducing reagent such as DTT and mercaptoethanol
