Product Description
| Download Documents | |
| SDS | Product Sheet |
| COA_Lot S356.S9.090421 | |
CellMosaic’s AqT® biotin antibody product is prepared by labeling the surface amines of the antibody with a super-hydrophilic AqT™ linker with exceptional high loading. AqT® linkers are novel proprietary biomaterials invented at CellMosaic® that are chemically assembled from a class of natural and edible sugar alcohol compounds with properties by design.
AqT® linker used to prepare this product is flexible with >20 atoms length. This long flexible hydrophilic spacer prevents the crowding and interaction of streptavidin with the antibody, increasing the sensitivity of detection.
Description of the Materials
- Product Name: AqT® Biotin G/H IgG, Fc gamma fragment specific
- Labeling Chemistry: Biotin is labeled at the surface amines of the antibody via a long and flexible super-hydrophilic AqT® linker (>20 atoms length)
- Molecule Weight: 154KDa
- Physical Form: White to off-white lyophilized powder in a centrifuge tube.
- Amount: each tube contains 100 μg of AqT® Biotin G/H IgG
Antibody Information
- Antibody Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non- immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
- Host/Isotype: Goat
- Class: Polyclonal
- Type: Secondary Antibody
Purity Specifications and Results
- Purity: Free of any unreacted AqT® biotin
- Purification Method: The antibody was purified by immunoaffinity chromatography. After biotinylation, the product was desalted to remove any unreacted AqT® biotin.
- Biotin Loading: average 4 to 6 per antibody (see COA for specific lot)
Usage
- Reconstitution: Dissolve in 100 μL of deionized water to obtain 1 mg/mL solution in 1xPBS buffer containing 2 mg/mL of BSA.
- Preservative: None
- Suggested Working Concentration or dilution Range (actual dilution used must be determined experimentally)
|
Applications |
Dilution (Suggested) |
|
ELISA and Western Blot (WB) |
1:20,000-1,400,000 |
|
Enzyme immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP) |
1:500-1:5,000 |
|
Flow Cytometry |
1:200-1:1,000 |
