Product Description
Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. BSA is commonly used as a carrier protein for immunization. This routine synthesis service is designed for the small-scale preparation of purified and characterized BSA–peptide conjugates with multiple peptides per BSA molecule. These multi-peptide–loaded BSA conjugates are suitable for immunization or detection applications and provide reliable performance. In addition, customers can select the desired peptide loading level based on their specific application needs.
Customers may either provide a Cys-containing peptide to CellMosaic or request CellMosaic to purchase the peptide if the sequence is provided. CellMosaic delivers the final conjugate along with reversed-phase and SEC-HPLC analysis data.
Customers can order online by providing peptide information and selecting any additional optional services.
What's included in the Base Price:
- Labor and materials (excluding Cys peptide; see Cys peptide requirements in the section below).
- Initial QC and quantitation of the starting peptide by reversed-phase C18 HPLC to confirm purity.
- In-process monitoring of BSA and peptide conjugation.
- Filtration and/or cartridge-type purification to remove the majority of unreacted peptides
- Final conjugate analysis by reversed-phase and SEC-HPLC to determine purity, concentration, and residual peptide.
- Use of sterilized buffers and plasticware throughout preparation.
Delivery: Final product is supplied in PBS buffer in a single tube, along with a COA that includes reversed-phase and SEC-HPLC data, concentration, and peptide loading.
Timeline: Approximately 2-3 weeks after receiving the starting peptide.
Selections and Add-Ons:
1. Reaction Scale: Choose between 5 mg and 10 mg BSA labeling scale. Typical recovery is 40–80%, depending on peptide properties. If you do not see the desired scale, please contact us for a custom quote.
2. Peptide Molecular Weight (MW): Choose between low (<3,000 Da), Medium (3,000–5,000 Da), and high (5,000–10,000 Da) molecular weight to ensure appropriate handling and reaction conditions. If you have a mini-protein or a peptide >10 kDa, please contact us for a quote.
3. Targeted Peptide Loading: Choose from three loading ranges based on your application needs:
- Low loading: 2–5 peptides per BSA
- Medium loading: 5–10 peptides per BSA
- High loading: >10 peptides per BSA
Please note: These ranges are for planning purposes only. No specific loading ratio is guaranteed unless the peptide has been synthesized previously at CellMosaic. Small peptides may achieve higher loading, while long or highly hydrophobic peptides usually result in lower-than-expected loading and are often supplied as low-loading even when high loading is requested.
4. Optional HPLC purification: HPLC purification is available to remove trace amounts of unreacted peptides. Recommended for high-MW or hydrophobic peptides.
5. Optional 0.2 µm Sterile Filtration: Routine preparation at CellMosaic uses sterilized buffers and pyrogen-free consumables. The final purified product is suitable for in vitro and in vivo studies. However, customers may request additional 0.2 µm sterile filtration after purification (note: this step may slightly reduce the final yield).
Requirements for Cys Peptide:
- Type: Standard peptide containing a reactive cysteine at either the N-terminus or C-terminus. If your peptide contains additional modifications, please contact us for a quote.
- Purity: HPLC-purified and lyophilized with ≥90% unoxidized Cys-peptide content by C18-HPLC. No additional components containing thiol groups (e.g. DTT) should be present.
- Amount:
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5 mg BSA labeling → 3 µmol (measured by weight) if high loading is requested. Unused materials can be returned upon request.
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10 mg BSA labeling → 6 µmol (Measured by weight) if high loading requested. Unused materials can be returned upon request.
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- Information: Provide as much detail as possible — amount, molecular weight, sequence, extinction coefficient — and attach QC data from your peptide vendor. Sequence information is required if CellMosaic is to order the peptide. The peptide should be non-toxic and non-hazardous to be ordered online. For toxic or highly toxic peptide conjugation, please contact us for a custom quote.
