Product Description
CellMosaic® has designed this Streptavidin-oligo conjugation kit with linkers and conjugation chemistry that retain the activity of Streptavidin.The same configuration and chemistry has been tested for Streptavidin-oligos with different oligo sequences by several different diagnostic companies since 2011.
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| SDS | USER MANUAL |
The kit is designed to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. Using the kit components, the user converts the amine-oligo to a thiol-oligo, followed by reaction of the thiol-oligo with activated Streptavidin to generate the Streptavidin-oligo conjugates. The total length of the linkage between the Streptavidin and the amine-oligo is 29 atoms excluding the linker used for amine modification of the oligo. The Q-column purification step typically provides the resulting Streptavidin-oligo at greater than 80% purity.
This kit provides materials to perform one or three strepatavidin and oligo conjugations. Scale of the reaction: 2 to 5 nmol oligo.
Note to previous users: If you purchased this kit previously, the current one is an upgraded and improved kit with slightly different components and workflow as the first and second-generation kit. This kit has multiple configurations for various lengths of oligos and can be used to conjugate 2−5 nano-mole (nmol) of oligo. Table 1 provides the catalog numbers for various kit configurations.
Table 1: Configurations of the PerKit™ Streptavidin-Oligo conjugation kit (CM52421)
| Configuration | No. of Reactions | Catalog No. |
| 1. Labeling for short oligo (5−30 bases) | 1 | CM52421.1x1 |
| 3 | CM52421.1x3 | |
| 2. Labeling for medium oligo (31−59 bases) | 1 | CM52421.2x1 |
| 3 | CM52421.2x3 | |
| 3. Labeling for long oligo (≥60 bases) | 1 | CM52421.3x1 |
| 3 | CM52421.3x3 |
Key Features of this Streptavidin-Oligo Conjugation Kit:
- High quality maleimide-activated streptavidin for single labeling.
- A single purification affords 85-90% single-labeled streptavidin-oligo conjugates if the quality of the oligo is at par.
- Preparation can be done in a day.
- Stable linkage with optimal spacer to ensure no interference between oligo binding and streptavidin activation.
- All reagents included, from preparation to purification.
- Options to choose tailored services at CellMosaic® after conjugation
After conjugation: you can choose to send your sample to CellMosaic® for size-exclusion chromatography (SEC) and anion exchange (AEX) HPLC analysis.
SEC HPLC separates the conjugates by apparent molecular weight (MW) or size in aqueous solution. The larger the MW of the conjugate, the earlier it elutes.
AEX HPLC separates the components according to the negative charges of the molecule.
You can also send your conjugates to CellMosaic for a complete removal of trace oligo impurities and unreacted streptavidin.
Labeling Chemistry
Typical Kit Performance Data (LC Analysis, CellMosaic)
Oligo information: HPLC-purified 23mer oligo with 5’ NH2-C6 modification
Kit lot number: 5528.s12.070119
Figure 1: SEC HPLC profiles of Activated Streptavidin (blue trace), oligo (red trace), and purified Streptavidin-Oligo Conjugate (E1) (green Trace) detected at 220 nm.
Table 1: Summary results
| Sample | Volume µL | % free Streptavidin | % free oligo | % of Streptavidin/oligo (1:1) |
| E1 (first elution) | 400 | Not detected | Not detected | >90% |
| Recovery: 95% of the conjugates | ||||
