Toggle menu
781-463-0002
  • LoginorSign Up
  • 0
CellMosaic
×
×

    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
    • Our Customers
    • Our Team
    • News
  • Contact Us
  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits
    • ADC Kits List
    • ADC Controls
    • ADC Analysis
    • Buffers and others
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Modified Biomolecules and Conjugates
    • Others
  • SERVICES
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • Mass Tags
  • OxLink™ and sxLink™ Protein crosslinkers
  • Shop By Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • Gift Certificates
  • Login or Sign Up
×

    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
    • Our Customers
    • Our Team
    • News
  • Contact Us
  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits
    • ADC Kits List
    • ADC Controls
    • ADC Analysis
    • Buffers and others
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Modified Biomolecules and Conjugates
    • Others
  • SERVICES
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • Mass Tags
  • OxLink™ and sxLink™ Protein crosslinkers
  • Shop By Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • Gift Certificates
  • Login or Sign Up

Shop by Category

  • Antibody-Drug Conjugate (ADC) Kits
    • ADC Kits List
    • ADC Controls
    • ADC Analysis
    • Buffers and others
  • AqueaTether™ (AqT™) Reagents and kits
    • AqT™ Biotin Labeling Kits and Products
    • AqT™ Fluorescence Labeling Kits and Products
    • AqT™ Immobilization Kits and Products
    • AqT™ Therapeutics
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Modified Biomolecules and Conjugates
    • Others
  • SERVICES
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • Mass Tags
  • OxLink™ and sxLink™ Protein crosslinkers
  • Shop by Brand

  • PerKit™
  • SepSphere™
  • oxLink™
  • sxLink™
  • View all Brands
  • CM53401x1 HRP Oligo Conjugation Kit Boxes
  • CM53401x1 HRP Oligo Conjugation Kit Boxes and content
  • Oligo HRP conjugate
  • HRP amine oligo conjugation scheme
  • CM53401x1 HRP Oligo Conjugation Kit Boxes CM53401x1 HRP Oligo Conjugation Kit Boxes and content Oligo HRP conjugate HRP amine oligo conjugation scheme

    HRP Oligo Conjugation Kit (Single Label with Amine Oligo)

    MSRP:
    Now: $302.00
    (You save )
    SKU:
    CM53401x1
    Availability:
    In stock, usually ships within 24 hrs
    Shipping:
    Calculated at Checkout

    Adding to cart… category.add_cart_announcement
    Add to Wish List
    • Create New Wish List
    • Email
    • Print
    • Linkedin
    • Overview

    Product Description

     

    Download Documents
    SDS USER MANUAL

    CellMosaic has designed this HRP-Oligo conjugate with linkers and conjugation chemistry that retain the activity of HRP. The same configuration and chemistry have been tested for HRP-Oligos with different oligo sequences in several diagnostic companies since 2011.

    This HRP-Oligo Conjugation kit works with amine-modified oligos. The customer supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. Using the kit components, the customer converts the amine-oligo to a thiol-oligo, followed by reaction of the thiol-oligo with activated HRP to generate the HRP-oligo conjugates. The Q-column purification step typically provides the resulting HRP-oligo at greater than 85% purity. 

    This kit provides materials to label one (CM53401x1) to three oligo samples (CM53401x3). Scale of the reaction: 5 nmol oligo.

    Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


    Key Features of this HRP-Oligo Conjugation Kit:

    • High quality maleimide-activated HRP for the conjugation: >99% purity and >200 units/mg protein activity 
    • Optimal maleimide groups per HRP for single label of oligo:  1.2 for typical batch
    • A single purification step affords 85~90% of single labeled HRP-oligo conjugates 
    • Fast preparation: less than 2 h hands-on time
    • Easy purification: less than 30 minutes
    • Stable linkage with optimal PEG spacer to make sure there is no interference between oligo binding and HRP activation
    • No activity loss of the  HRP after the conjugation and purification
    • All reagents included, from preparation to purification

     Labeling Chemistry

    scheme.jpg

     

     


    Typical Kit Performance Data (LC Analysis, CellMosaic)

    Oligo information: HPLC-purified 18mer oligo with 5’ NH2-C6 modification

    Figure 1: Size exclusion HPLC analysis of the HRP and oligo reaction mixture (before purification).

    figure1-cm53401.jpg

     

    Figure 2: Size exclusion HPLC analysis of first elution (E1) from Column Q (after purification).

    figure2-cm53401.jpg

    Product Videos

    Custom Field

    Product Reviews

    Recommended

    • HRP-oligo conjugate through thiol ether formation HRP thiol oligo conjugation scheme HRP-oligo conjugate through thiol ether formation HRP thiol oligo conjugation scheme
      Quick view Details

      PerKit™

      |

      sku: CM53402x1

      HRP Oligo Conjugation Kit (Single Label with Thiol Oligo)

      MSRP:
      Now: $302.00
      Choose Options
    • HRP antibody conjugate HRP antibody conjugate HRP antibody conjugate HRP antibody conjugate
      Quick view Details

      PerKit™

      |

      sku: CM51406x1

      HRP Antibody Conjugation Kit

      MSRP:
      Now: $620.00
      Choose Options
    • Peptide oligo conjugation kit CM42301 Peptide oligo conjugation kit CM42301
      Quick view Details

      PerKit™

      |

      sku: CM42301

      Perkit™ peptide-oligo conjugation kit

      MSRP:
      Now: $582.00
      Choose Options
    • Perkit™ Antibody-Oligo Conjugation Kit Schematic diagram of the workflow for preparing antibody–oligo conjugates starting with 1 mg of antibody Perkit™ Antibody-Oligo Conjugation Kit Schematic diagram of the workflow for preparing antibody–oligo conjugates starting with 1 mg of antibody
      Quick view Details

      PerKit™

      |

      sku: CM53405

      Perkit™ Antibody-Oligo Conjugation Kit

      MSRP:
      Now: $582.00
      Choose Options
    • Chemical Structure of Streptavidin Oligo Conjugate Scheme 1: Preparation of 1:1 Streptavidin Oligo Conjugate Chemical Structure of Streptavidin Oligo Conjugate Scheme 1: Preparation of 1:1 Streptavidin Oligo Conjugate
      Quick view Details

      sku: CM52421x1

      Streptavidin Oligo Conjugation Kit (Single Label with Amine Oligo)

      MSRP:
      Now: $683.00
      Choose Options
    ×
    ×
    Join Our Mailing List for special offers!
    Contact Us
    10A Roessler Road
    Woburn, MA 01801
    781-463-0002
    info@cellmosaic.com
    Accounts & Orders
    • Wishlist
    • Login or Sign Up
    Quick Links
    • About Us
    • Contact Us
    • Products and Services
    • How to Order
    • Technologies
    • Resources
    • Careers
    Connect with Us:
    • © CellMosaic
    • |
    • Sitemap