Product Description
CellMosaic® has designed this PerKit™ HRP−Oligo conjugation kit to prepare single-labeled HRP−Oligo conjugates with linkers and conjugation chemistry that retains the activity of HRP. The same configuration and chemistry have been tested for HRP-oligos with different oligo sequences at a few different diagnostic companies since 2011.
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The kit is designed to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. Using the kit components, the user converts the amine-oligo to a thiol-oligo, followed by reaction of the thiol-oligo with activated HRP to generate the HRP-oligo conjugates. The total length of the linkage between the HRP and the amine-oligo is 29 atoms excluding the linker used for amine modification of the oligo. The Q-column purification step typically provides the resulting HRP-oligo at greater than 80% purity.
This kit is an upgraded and improved replacement kit with slightly different components and workflow as the first-generation kit (Cat# CM53401). This kit has multiple configurations for various lengths of oligos and can be used to conjugate 1-5 nano-mole (nmol) of oligo. Table 1 provides the catalog numbers for various kit configurations.
Table 1: Configurations of the PerKit™ HRP−Oligo conjugation kit (CM53407)
|
Configuration |
No. of Reactions |
Catalog No. |
|
1. Labeling for short oligos (5-30 bases) |
1 |
CM53407.1x1 |
|
3 |
CM53407.1x3 |
|
|
2. Labeling for medium oligos (31-59 bases) |
1 |
CM53407.2x1 |
|
3 |
CM53407.2x3 |
|
|
3. Labeling for long oligos (≥60 bases) |
1 |
CM53407.3x1 |
|
3 |
CM53407.3x3 |
Requirement for amine-oligo:
- Amount: 1-5 nano-mole (nmol) oligo content as measured by UV
- HPLC purified and lyophilized oligo with amine-oligo content ≥90% by C18 HPLC
- ≥5- bases (oligos longer than 60 bases may result in lower loading and high impurities).
Key Features of this HRP-Oligo Conjugation Kit:
-
High quality maleimide-activated HRP for the conjugation: >99% purity and >200 units/mg protein activity.
-
A single purification step affords 85~90% of single labeled HRP-oligo conjugates if the quality of the oligo is at par.
-
Preparation can be done in a day.
-
Stable linkage with optimal spacer to make sure there is no interference between oligo binding and HRP activation.
-
All reagents included, from preparation to purification.
- Options to choose tailored services at CellMosaic® after conjugation:
- You can choose to send your conjugates to CellMosaic® for HPLC analysis of the sample or complete removal of trace oligo impurities and unreacted HRP.
Labeling Chemistry
