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  • PerKit™ HRP−Oligo Conjugate
  • PerKit™ HRP−Oligo Conjugation Workflow
  • PerKit™ HRP−Oligo Conjugate PerKit™ HRP−Oligo Conjugation Workflow

    HRP Oligo Conjugation Kit (Single Label with Amine Oligo) (Replacement)

    PerKit™

    MSRP:
    Now: $332.00
    (You save )
    SKU:
    CM53407
    Availability:
    In stock, usually ship within 1 week.
    Shipping:
    Calculated at Checkout

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    Product Description

     

    Download Documents
    SDS USER MANUAL

    CellMosaic® has designed this PerKit™ HRP−Oligo conjugation kit to prepare single-labeled HRP−oligo conjugates with linkers and conjugation chemistry that retains the activity of HRP. The same configuration and chemistry have been tested for HRP-oligos with different oligo sequences at a few diagnostic companies since 2011. 

    The kit is designed to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. Using the kit components, the user converts the amine-oligo to a thiol-oligo, followed by reaction of the thiol-oligo with activated HRP to generate the HRP-oligo conjugates. The total length of the linkage between the HRP and the amine-oligo is 29 atoms excluding the linker used for amine modification of the oligo. The Q-column purification step typically provides the resulting HRP-oligo at greater than 80% purity. 

    This kit is an upgraded and improved replacement kit with slightly different components and workflow as the first-generation kit (Cat# CM53401). This kit has multiple configurations for various lengths of oligos and can be used to conjugate between 1 to 5 nano-mole (nmol) of oligo. Table 1 provides the catalog numbers for various kit configurations and Table 2 lists the kit components.

    Table 1: Configurations of the PerKit™ HRP−Oligo conjugation kit (CM53407)

    Configuration

    No. of Reactions

    Catalog No.

    1. 1.    Labeling for short oligo

    (5-30 bases)

    1

    CM53407.1x1

    3

    CM53407.1x3

    1. 2.    Labeling for medium oligo

    (31-59 bases)

    1

    CM53407.2x1

    3

    CM53407.2x3

    1. 3.    Labeling for long oligo

    (≥60 bases)

    1

    CM53407.3x1

    3

    CM53407.3x3


    Key Features of this HRP-Oligo Conjugation Kit:

    • High quality maleimide-activated HRP for the conjugation: >99% purity and >200 units/mg protein activity. 
    • A single purification step affords 85~90% of single labeled HRP-oligo conjugates if the quality of the oligo is at par.
    • Preparation can be done in a day.
    • Stable linkage with optimal spacer to make sure there is no interference between oligo binding and HRP activation.
    • All reagents included, from preparation to purification.
    • Options to choose tailored services at CellMosaic after conjugation:
      • You can choose to send your conjugates to CellMosaic for HPLC analysis of the sample or complete removal of trace oligo impurities and unreacted HRP.

     Labeling Chemistry

    scheme-1-hrp-oligo-kit-cm53407.png

     

     

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