Product Description
| Download Documents | |
| SDS | USER MANUAL CM86248 (1-3 mg scale) |
| USER MANUAL CM86248.01 (0.1 mg scale) | |
AqT® Fluor 750 Antibody Labeling kit is a proprietary fluorescent labeling kit developed at CellMosaic® for obtaining high quality and high loading of fluorescent labeled antibody with minimal or no aggregation.
Commercial fluorescent dyes are often highly hydrophobic, so dye-labeled antibodies can aggregate and exhibit fluorescence quenching at high dye loading due to fluorophore stacking, reducing signal. The AqT® Fluor 750 Antibody Labeling Kit (Surface Amines) uses CellMosaic’s super-hydrophilic, water-soluble, charge-neutral AqT® linker to improve dye-labeled antibody performance by increasing solubility, reducing aggregation and nonspecific binding, and minimizing quenching; it employs a core dye with Ex/Em 750/780 nm, and the AqT® spacer helps maintain antibody function and produce brighter, more stable conjugates with improved signal intensity.
For a detailed description of the AqT® technologies, please see our technology section.
The CM86248 kit is designed to label any antibody with AqT® Fluor 750 dye via surface amines on the antibody. The user supplies the antibody. The kit includes AqT® Fluor 750 acid, which is activated within 15 minutes and then coupled directly to the antibody in one step. The labeled product is subsequently purified to remove any unreacted AqT® Fluor 750 acid
The kit is available in multiple configurations for labeling antibodies ranging from 0.1 to 3 mg. Each configuration supports labeling of either one (×1) or three (×3) antibody samples. Please refer individual configuration to download the user manual.
|
Catalog Number |
Number of Reactions in Kit |
Antibody Amount per Reaction |
|
CM86248.01x1 |
1 |
100 μg (0.667 nmol) |
|
CM86248.01x3 |
3 |
|
|
CM86248.1x1 |
1 |
0.4-1 mg (2.67-6.67 nmol) |
|
CM86248.1x3 |
3 |
|
|
CM86248x1 |
1 |
1-3 mg (6.67-20 nmol) |
|
CM86248x3 |
3 |
If you have any other customization needs, please provide the details above.
Requirement for Antibody
- MW: ≥ 100 KDa (if MW is lower, please use an alternative kit)
- Preferably >90% pure by gel electrophoresis
- Total amount: 0.1 mg or 0.4-3 mg of protein content as measured by UV.
Key Features
- Optimized for near-infrared imaging: Features a Fluor 750 core dye (Ex 750 nm / Em 800 nm), ideal for IR-range applications.
- Advanced AqT® linker technology: Utilizes CellMosaic’s proprietary, super-hydrophilic, water-soluble, charge-neutral AqT® linker to minimize fluorophore stacking and antibody aggregation.
- High dye loading with preserved antibody function: Typical degree of labeling (DOL) of 3–5 dyes per antibody while maintaining antibody activity. No increase in apparent MW (hydrodynamic volume) or aggregation.
- All-inclusive kit with validated analytical method: Provides sterilized buffers and tubes, columns, and filters for fluorescent labeling. Also includes all buffers and methods needed for concentration measurement and loading determination.
- Fast, user-friendly workflow plus optional support: One-step labeling and purification in ~2 hours (≤1 hour hands-on), with optional post-conjugation services at CellMosaic® for analytical characterization and DOL determination.
Labeling Chemistry
Protocol
Schematic workflow diagram for preparing AqT® Fluor 750 labeled antibody within 2 hours, starting with 3 mg of IgG. (Reagent volume will vary if the amount of IgG is less than 3 mg).
Other Details
The information in this document is subject to change without notice. CellMosaic® assumes no responsibility for any errors or omissions that may appear in this document. Under no circumstances shall CellMosaic be liable, whether in contract, tort, warranty, or under any statute or on other legal theory, for special, incidental, indirect, punitive, multiple, or consequential damages related to or arising from this document, including but not limited to the use of this product thereof.CellMosaic’s products, including but not limited to AqT®, NeIon™, oxLink™ and sxLink™ (collectively, the “Products”), are covered by, or are the subject of, one or more issued patents and pending patent applications, including without limitation: 9,688,663 B2; 8,907,079 B2; 9,511,150 B2; 9,907,854 B2; 9,346,833 B2; 9,518,067 B2; CN104066451B; CN107043339B; CA 2,841,313; EP 2734238 B1; AU2012284055; JP 6240599 B2; and corresponding foreign patents and patent applications. The purchase of the Product conveys solely a limited, non-exclusive, non-transferable, non-sublicensable license to use the Product only for the purchaser’s internal research and development purposes. No right or license is granted, either expressly or by implication, estoppel, or otherwise, under any patent, trademark, copyright, or other intellectual property right of CellMosaic, except as expressly stated herein. Any commercial use of the Product is strictly prohibited without a separate written agreement from CellMosaic. Prohibited commercial uses include, without limitation: (a) the sale, lease, licensing, distribution, or other transfer of the Product or any materials derived from or produced using the Product; (b) the sale, lease, licensing, or other grant of rights to use the Product or any materials derived from or produced using the Product; and (c) the use of the Product to perform services for a fee or other consideration for third parties, including without limitation contract research, screening services, or diagnostic or therapeutic applications. For information regarding commercial licensing or collaboration, please contact us at info@cellmosaic.com.
