Product Description
Horseradish peroxidase (HRP) is is an oxidoreductase enzyme isolated from the roots of the horseradish plant (Armoracia rusticana) and catalyzes the oxidation of various substrates using hydrogen peroxide (H₂O₂) as an electron acceptor. It is widely used in biochemical, diagnostic, and analytical applications because of its high stability, strong catalytic activity, and compatibility with many detection systems. CellMosaic® is a preferred choice for diagnostic companies to source highly pure and single-labeled HRP-oligo conjugates.
This routine synthesis service is designed for the small-scale preparation of purified and characterized HRP–oligo conjugates, with one oligo per HRP. This single-labeled conjugate is especially suitable for diagnostic applications with reliable performance.
An amine-modified oligo can be supplied by the customer or purchased by CellMosaic if the sequence is provided. CellMosaic delivers the final conjugate with SEC-HPLC analysis data.
Customers can order online by providing oligo information and selecting from various reaction scales, linker types (stable or releasable), and additional optional services.
What's included in the Base Price:
- Labor and materials (excluding antibody and oligo).
- Initial QC and quantitation of the starting oligo by C18 HPLC to check the purity.
- In-process monitoring of the oligo modification and HRP-oligo conjugation.
- Cartridge-type purification to remove any unreacted HRP.
- Final product analysis by AEX HPLC to determine purity, aggregation profile, concentration, percentage of unreacted antibody, residual oligo.
Delivery: Final product is supplied in Tris buffer in a single tube along with a COA that includes SEC-HPLC data, concentration, and oligo loading.
Timeline: Approximately 2-3 weeks after receiving the starting antibody and oligo.
Selections and Add-Ons:
1. Reaction Scale: Choose between 10 nmol and 50 nmol oligo labeling scale. Typical recovery is 30-70%, depending on the oligo properties. If you do not see the desired scale, please contact us for a custom quote.
2. Oligo Length: Please indicate the number of nucleotides in your oligo so that appropriate handling and conditions can be applied.
3. Optional AEX Analysis: AEX-HPLC provides charge-based analysis of AOCs and often offers better resolution. If conjugate peaks are separable, AEX can be used to calculate overall oligo loading. Two samples are generally required for this analysis (oligo and conjugate). [Click here for more information].
4. Optional AEX HPLC purification: For project requires complete removal of unreacted oligo and HRP, AEX HPLC is highly effective. After purification, conjugate will be reanalyzed by SEC HPLC to determine the concentration.
Requirements for Amine Oligo:
- Type: Standard amine DNA oligo with deoxyribose–phosphate backbone. For modified backbones such as phosphorothioate (PS), locked nucleic acids (LNA), RNA, or morpholino oligos, please contact us for a quote.
- Purity: HPLC-purified and lyophilized amine oligo with ≥90% amino-oligo content by C18-HPLC. No additional components containing primary/secondary amines or thiol groups should be present.
- Amount: 15 or 30 nmol oligo (3 x amount of choosing scale) (Measured by UV.) Unused materials can be returned upon request
- Information: Provide as much detail as possible — amount, molecular weight, sequence, extinction coefficient — and attach QC data from your oligo vendor. Sequence information is required if CellMosaic is to order the oligo.
