Product Description
Size exclusion chromatography (SEC) HPLC is suitable for the analysis of mixtures of biopolymers. It separates components based on their apparent molecular weight (MW) or size in aqueous solution, with larger molecules eluting earlier than smaller ones. This method can be used to estimate the approximate molecular weight of analytes.
For bioconjugate analysis, such as antibody–drug conjugates (ADCs), SEC HPLC is particularly useful for assessing the presence of unconjugated species and evaluating the degree of aggregation within a sample.
Following analysis at CellMosaic, a standard PDF report of the HPLC profiles will be provided.
Pricing is per sample. A $250 setup fee applies per batch analysis and will be waived if the conjugate is prepared using a CellMosaic kit or if an extended turnaround time (up to 9 business days) is acceptable. Standard turnaround time is typically 1–3 business days.
Sample submission for biopolymers, bioconjugates, ADCs:
1) For biopolymers, bioconjugates, and ADCs, dilute samples in PBS buffer to 0.5–2 mg/mL. Centrifuge (≥5000 × g) to remove particulates. Transfer a minimum of 50 μL of the supernatant to a 500 μL screw-cap microcentrifuge tube.
2) Please download and complete the Sample Submission Form with all required information. Email a copy, and include a printed copy with your samples. Ship with a cold pack via overnight or earliest delivery.
For analysis of bioconjugates such as antibody drug conjugates, always ship a sample of un-labeled biopolymer for analysis at the same time.
Related Products:
SEC HPLC Protein Standards (CM92004 and CM92005): CellMosaic offers two SEC standards for our customers to use with any SEC column. Each standard is designed for day to day use to check HPLC column performance and for the analysis of bioconjugates. The CM92004 and 92005 product sheets contain all of the information and methodology you need to run an SEC HPLC analysis.
