Product Description
Size exclusion chromatography (SEC) HPLC is suitable for analyzing mixtures of biopolymers. SEC HPLC separates bioploymers by their apparent molecular weight (MW) or size in aqueous solution. The larger the MW of the bioploymer, the earlier it elutes. It can be used to determine the approximate molecular weight of the molecules. In the case of bioconjugate analysis, such as antibody drug conjugate (ADC), it can be used to assess the amount of unlabeled molecules and degree of bioconjugate aggregation in a sample. After submitting samples to CellMosaic for analysis, a standard pdf file of the HPLC profiles will be supplied.
Per sample charge.
Setup fee of $250 applies per batch analysis. Setup fee will be waived if the conjugate is made using CellMosaic's kit or if sample can be analyzed within a longer timeframe of up to 7 business days. Standard service is 1-3 business days.
Sample submission for biopolymers, bioconjugates, ADCs:
1) Dilute your samples in PBS buffer to 1 mg/mL, and then transfer minimum 50 μL of the diluted solution to a 500 μL micro-centrifuge tube. Label the vial properly.
2) Ship your samples with a cold pack for overnight delivery.
For analysis of bioconjugates such as antibody drug conjugates, always ship a sample of un-labeled biopolymer for analysis at the same time.
Related Products:
SEC HPLC Protein Standards (CM92004 and CM92005): CellMosaic offers two SEC standards for our customers to use with any SEC column. Each standard is designed for day to day use to check HPLC column performance and for the analysis of bioconjugates. The CM92004 and 92005 product sheets contain all of the information and methodology you need to run an SEC HPLC analysis.
