Product Description
This HPLC method is designed for the analysis of oligonucleotides (DNA and RNA) and their conjugates. C18 HPLC separates components based on length and hydrophobicity, enabling effective characterization of sample composition. Retention time shifts can be used to determine whether an oligo has been modified.
Following analysis at CellMosaic, a standard PDF report of the HPLC profiles will be provided.
Pricing is per sample. A $100 setup fee applies per batch analysis and will be waived if the conjugate is prepared using a CellMosaic kit or if an extended turnaround time (up to 9 business days) is acceptable. Standard turnaround time is typically 1–3 business days.
Sample submission for oligos (DNA, RNA) and their conjugates:
1) For oligonucleotides (DNA, RNA) and their conjugates, dilute samples in water to 10–100 μM. Centrifuge (≥5000 × g) to remove particulates. Transfer a minimum of 50 μL of the supernatant to a 500 μL screw-cap microcentrifuge tube.
2) Please download and complete the Sample Submission Form with all required information. Email a copy, and include a printed copy with your samples. Ship with a cold pack via overnight or earliest delivery.
For analysis of modified oligo and conjugate, always ship an un-labeled oligo for analysis at the same time.
