This HPLC method is suitable for analyzing protein and modified protein with a hydrophobic small molecule, separating the components according to hydrophobicity. It can be used to determine if a protein is modified or not based on the retention time change.
After submitting samples to CellMosaic for analysis, a standard pdf file of the HPLC profiles will be supplied.
Per sample charge.
Setup fee of $100 applies per batch analysis. Setup fee will be waived if the conjugate is made using CellMosaic's kit or if sample can be analyzed within a longer timeframe of up to 7 business days. Standard services is 1-3 business days.
Sample submission for protein and their conjugate:
1) Dilute your samples in water to 1 mg/mL, and then transfer minimum 50 μL of the diluted solution to a 500 μL micro-centrifuge tube. Label the vial properly.
2) Ship your samples with packing material and shipping method that are suitable for your samples.
For analysis of conjugate, always ship a sample of un-labeled small molecule and protein for analysis at the same time.