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Solid Support
SepSphere™ Immobilization Kits
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| TECHNIQUE NOTE |
Biopolymers and small molecules immobilized on solid support are important reagent-tools for biotech research. An antigen immobilized on a solid support, such as agarose bead, magnetic bead, or polymer bead is often used as affinity purification media. Immobilized antigen can also be used as a capture tool for protein/marker identification in drug discovery or as a diagnostic reagent in immunoassay. The development of techniques and methods for immobilization is essential for many biotechnological advancements.
Two types of solid supports are developed at CellMosaic
- Conventional hydrophilic agarose beads functionalized with cyanate esters or imidocarbonates (CNBr activated), amino, carboxylic acid, glyoxy, glutaldehyde, aminooxy, pyridyl disulfide, maleimide, or epoxide for the convenience of immobilization using the customer’s own compound
- Novel hydrophilic AqT™ coated beads.
Three Essential Components of a Successful Immobilization Kit
- Quality and stability of the functionalized beads - CellMosaic has tailored the loading capacity and stability of the agarose beads for each application.
- Chemistry design - CellMosaic’s kit comes with pre-made buffers with simple easy to follow direction.
- Immobilization workflow - We have developed a universal workflow and kit design that allows immobilization, washing of unreacted starting materials, and column packing all in one column with a simple set-up in any lab. The immobilization efficiency in the column is the same as if performed in a regular reaction vessel. The washing is performed with a simple manual air push mechanism using a syringe and requires no gas/air flow or vacuum. For more information, please click here.
CellMosaic currently provides the following SepSphere™ immobilization kit using agarose beads, and we are adding additional kits configurations as needed. Please inquire if you do not see your current configuration of interest listed or request a custom immobilization service if you are interested in using a solid support other than agarose beads.
SepSphere™ Immobilization Kits Selection Guide
Product# |
Ligand |
Ligand Reactive Groups |
Agarose Reactive Groups |
Linkage |
Stability of Linkage |
| CM71401 | Oligo, DNA | -NH2 | -COOH | Amide bond | Stable |
| CM71402 | Oligo, DNA | -NH2 | -CH(O)CH2 (epoxide) | β-amino alcohol | Stable |
| CM71403 | Oligo, DNA | -OPO3 (phsophate) | -COOH | Amide bond | Stable |
| CM71004 | Small molecule | -COOH | -NH2 | Amide bond | Stable |
| CM71005 | Small molecule | -NH2 | -C(=O)H (glyoxyl) | Secondary amine | Stable |
| CM71006 | Small molecule | -NH2 | -CH(O)CH2 (Epoxide) | β-amino alcohol | Stable |
| CM71007 | Small molecule | -OH (Aliphatic alcohol) | -NH2 | Thiol ether | Stable |
| CM71008 | Small molecule | -NH2 | -COOH | Amide bond | Stable |
| CM71010 | Small molecule | -C=O- (Ketone or aldehyde) | -ONH2 (aminooxy) | Oxime bond | Stable (can be released at acidic or basic conditions) |
| CM71301 | Peptide | -SH (Cys thiol) | Maleimide | Thiol ether | Stable |
| CM71302 | Peptide | -SH (Cys thiol) | -S-S-Pyridine | Disulfide bond | Stable (can be released under reducing conditions) |
| CM71303 | Peptide | -NH2 | -COOH | Amide bond | Stable |
| CM71542 | Antibody/ Protein/Enzyme | -NH2 | -C(=O)H (glyoxyl) | Secondary amine | Stable |
| CM71543 | Antibody/ Protein/Enzyme | -NH2 | Glutaraldehyde | Imine | Stable at pH 5-9 |
AqueaTether® (AqT®) TechnologyEven with sugar-based agarose beads, we experience challenges obtaining high loading of antigen molecules with conventional linkers. When we do manage to achieve high loading, beads aggregate or cluster together, making them difficult to use. AqT® modification can help to reduce the hydrophobicity added by high loading of antigen molecules. CellMosaic is currently developing next-generation AqT®-modified beads and immobilization kits/products. Learn morea bout AqT® technologies. If you are interested in co-developing AqT® beads and immobilization kits, click here to learn about partnering with CellMosaic. |