ADC Control hIgG1-MMAE

Product Description

MMAE (monomethyl auristatin E) is an antimitotic agent that binds to tubulin and inhibits the assembly of microtubules. Mc-VC-PAB-MMAE is a thiol reactive derivative of MMAE that contains a cathepsin B cleavable valine-citruline p-aminobenzylcarbamate linkage (VC-PAB). Mc-VC-PAB-MMAE is used to prepare antibody-drug conjugates, such as brentuximab vedotin (Seattle Genetics).

This product is a control ADC for any IgG type antibody labeled with MMAE, particularly any ADC made using CellMosaic's Antibody MMAE Conjugation Kit (Cat#: CM11409). The product uses a human IgG1 subtype antibody that does not bind to the target. It has an average of 3-5 MMAE loaded on each antibody and can be used for accessing the effect of MMAE alone (non-specific toxicity). The product uses a very low endotoxin level antibody as a starting material (≤0.5 EU/mg by the LAL method). The preparation and purification are conducted under pyrogen free environment. The final product is assayed for endotoxin to make sure it is suitable for cell culture and animal studies. The loading is confirmed by various methods including UV, HPLC, and difference MALDI-TOF MS.

The final conjugate is lyophilized from phosphate buffered saline containing sugar-based stabilizer for easy shipping and storage. 

The product is sold as 1 vial of 100 microgram (Cat# CM51100-100UG), 1 vial of 500 micrograms (Cat# CM51100-500UG), or 2 vials of 500 micrograms (Cat# CM51100-1MG). For bulk orders, please contact us for a quote.

Chemical Information

• Chemical name: ADC Control hIgG1-MMAE
• Chemical formula: N/A
• Molecular weight: 152KDa           CAS number: N/A

 Specification (current COA)

• Physical Appearance: Colorless to white lyophilized powder in a microcentrifuge tube
• Storage Temp.: -20°C
• Purity: ≥99% of MMAE labeled antibody by SEC HPLC, free of any unreacted MMAE
• Endotoxin Level: ≤0.5 EU/mg by the LAL method
• Drug Over Antibody Ratio (DAR): ~4 by UV ratio, HIC HPLC, and difference MALDI-TOF MS

Antibody Information

• Immunogen: HEL (Hen Egg Lysozyme) 
• Species Reactivity: chicken
• Expression Host: CHO-S cell line
• Class: Human IgG1, Kappa
• Antibody Type: Monoclonal
• Applications: ELISA, FACS, IF, IHC, in vivo studies, IP, WB
• Purification Method: Affinity purified

Frequently Asked Questions:

The list below includes FAQs that are specific to this product.

Question: Do you have any references concerning this product, especially the concentration to use. What would be an optimal concentration to use as a control?

Answer: We don’t have any references for this product because it is a new product made by us. As a general rule, for any control usage, you should use the same drug concentration as your binding ADC (after taking into consideration the DAR). The concentration of binding ADC you use will depend on your application. For cell viability studies to determine the IC50, you can use various concentrations ranging from 0.001 µg/mL to 10 µg/mL.

Question: What is so special about your control product? Can we use it for in vivo studies?

Answer: Our control ADC is produced using the same method as CM11409 (our kit) to enable direct head-to-head comparison. At CellMosaic, we provide the highest quality bioconjugate products with full characterization to support customers’ in vitro and in vivo studies. The control hIgG1 is sourced from a well-known company and is specifically designed for in vivo studies, featuring a low endotoxin level. The final product undergoes extensive characterization, including HIC-HPLC, SEC-HPLC, and MALDI-TOF MS. Each batch is tested to ensure an endotoxin level below 0.5 EU/mg.

If you can’t find the answer you’re looking for or need information on general topics, please visit the main Frequently Asked Questions (FAQs) section.

Related Products

Antibody MMAE Conjugation Kit (With VC-PAB Linkage) (Product #: CM11409): ADC kit designed for labeling any IgG type antibody with MMAE using a cleavable VC-PAB linker for early-stage screening of customer's antibody.