SepSphere™ Alprenolol Agarose

Alprenolol is an antagonist for ß1 and ß2 adrenergic receptors. Alprenolol agarose affinity chromatography was first introduced by Lefkowitz R. J. in 1979 (References 1 and 2) for the purification of erythrocyte ß-adrenergic receptor based on ligand-receptor interactions. It is now routinely used for the purification of functionally active ß1 and ß2-adrenergic receptor. CellMosaic’s SepSphere™ Alprenolol Agarose is composed of Agarose 4B Beads linked to alprenolol via a thiol ether linkage.  

Chemical Information

 Alprenolol immobilized on agarose 4B beads through thiol ether linkage.

Specification

• Ligand: alprenolol
• Physical appearance: suspension in 0.02% NaN3 solution.
• Immobilization method: immobilized through the reaction of the double bond of alprenolol and S-alkyl-thiosulfate agarose.
• Storage temp.: 2- 8 °C
• Ligand concentration: 1 ~ 3 uMole per mL of settled beads by thiol assay
• Matrix: agarose 4B (source: GE Life Science).
• Spacer: 12 atoms
• Particle size: 45-165 um

 References

1. Shorr, R., G.; Lefkowitz, R. J.; Caron, M. G. Purification of the ß-adrenergic receptor. J. Bio. Chem. 1981,  256(11),  5820-5826;
2. Caron, M. G.; Srinivasan, Y.; Pitha, J.; Kociolek, K.; Lefkowitz, R. J. Affinity chromatoraphy of the ß-adrenergic receptor J. Bio. Chem. 1979,  254(8),  2923-2927.

Citations from Customer Who Used Our Product:

Salom, D.; Padayatti, P. S.; Palczewski, K. Crystallization of G protein-coupled receptors Methods Cell Biology 2013, 117, 451-468. DOI: 10.1016/B978-0-12-408143-7.00024-4

Cheong, H. I.; Asosingh, K.; Stephens, O. R.; Queisser, K. A.; Xu, W.; Willard, B.; Hu, B.; Dermawan, J. K. T.; Stark, G. R.; Naga Prasad, S. V.; Erzurum, S. C. Hypoxia sensing through β-adrenergic receptors JCI Insight 2016, 1(21), e90240. DOI: 10.1172/jci.insight.90240

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