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Streptavidin/Avidin Labeling and Conjugation

Streptavidin is a tetrameric protein containing four biotin-binding sites, one on each subunit. The streptavidin/avidin-biotin system is one of the most widely used affinity-based technologies in biochemistry, molecular biology, diagnostics, and bioconjugation. The avidin/streptavidin-biotin interaction is among the strongest non-covalent interactions known in nature, with a dissociation constant (Kd) of approximately 10^-14 to 10^-15 M. 

Because biotin can be readily introduced into proteins, antibodies, peptides, oligonucleotides, and other biomolecules, streptavidin serves as a versatile molecular connector and enables highly modular assay and conjugation systems. Major applications of the streptavidin/avidin-biotin system include ELISA assays using streptavidin-HRP detection, immunohistochemistry (IHC), nucleic acid detection, affinity purification, immobilization on streptavidin-coated surfaces, and signal amplification technologies.

At CellMosaic, we have extensive experience performing custom bioconjugation projects involving streptavidin, avidin, and monovalent streptavidin. We have successfully prepared a wide range of single-labeled and multi-labeled bioconjugates for research, diagnostic, and therapeutic applications.

• Labeled streptavidin/avidin reagents—please click here.
• PerKit® for streptavidin/avidin labeling and conjugation—please click here.
• For large scale or project beyond the scope of PerKit™ configuration or our routine synthesis, please contact us for a quote.