Solid Support - Custom Services

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CellMosaic provides comprehensive immobilization services for attaching biopolymers and small molecules onto solid supports such as agarose, dextran gels, glass beads/plates, and functionalized resins/beads. Available functional groups include amine, carboxyl, hydroxyl, aminooxy, hydrazine, thiol, keto, and aldehyde. Common biopolymers we work with include proteins, peptides, and oligonucleotides, as well as small molecules such as ligands, haptens, and therapeutic compounds.

Our solid support labeling products are offered under the SepSphere™ brand—please click here.
For custom immobilization services, please contact us for a quote.

Example 1: High-Loading, High-Specificity Antibody-Binding Protein A Agarose Beads Prepared at CellMosaic Using a Cyanogen Bromide Activation Method

Ligand density: ≈ 5 mg protein A/ml drained medium

Available binding capacity: ≈ 42 mg human IgG/ml drained medium

Binding specificity: only hIgG is bind to the Protein A agarose beads

SDS PAGE Result: A 5 µl samples was run on a 4-12% Bis-Tris SDS-PAGE gel (Invitrogen) uisng 1 X MES running buffer and stained with SimplyBlue (Sigma).

Checking Specificity of Protein A Agarose Prepared at CellMosaic

I (Input): Input mixture ( 40 µL PBS buffer containing 1 µg/µL human IgG, and 0.5 µg/µL each thyroglobumin, BSA, carbonic anhydrase, and RNase A).
A (Sample): Supernatants from the input mixture after incubation with 10 µl of Protein A agarose (settled beads volume) at room temperature for 2 hours.
B (Control): Supernatants from the input mixture after incubation with the control agarose beads (without immobilized Protein A) at room temperature for 2 hours.

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