KLH-Peptide Conjugation Kit

Product Description

Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, our customers prepare the KLH-peptide conjugate by reacting their peptide (supplied by the customer) with activated KLH. A single step purification process then typically provides the resulting KLH-peptide conjugate at greater than 95% purity.

This kit provides materials to perform labeling of KLH with one (CM52411x1) or three (CM52411x3) peptides. Total amount of activated KLH included in a reaction: 2 mg.

Requirement for Cys peptide:

• Amount: 0.10–0.51 µmol (20–100 equivalents of peptide over KLH)
• HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
• The Cys peptide should be stored at -80°C.
• HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

Key Features of this KLH-Peptide Conjugation Kit:

• High quality maleimide-activated KLH for the conjugation: >95% purity 
• High maleimide groups per KLH for peptide loading: 40-100
• A single purification step affords over 95% of the KLH-peptide conjugates 
• Fast preparation: less than 1 hour hands-on time
• All reagents included from preparation to purification
• Options to choose tailored services at CellMosaic® prior to and after conjugation

Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic® will give recommendations for the conjugation if your peptide has special features (complementary service);

After conjugation: you can choose to send your sample to CellMosaic® for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.