Product Description
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R-Phycoerythrin (PE) is an extremely bright red-orange fluorescent dye isolated from red algae. R-PE-Streptavidin conjugates are commonly used for detecting biotinylated antibodies and other biotinylated molecules in fluorescence-based assays, such as flow cytometry, microarray assays, and ELISAs. R-PE conjugates often provide 5 to 10 times greater sensitivity in these applications compared to fluorescein-labeled conjugates. The quality of the conjugate and linker significantly affects background levels and assay sensitivity.
CellMosaic’s R-PE-Streptavidin conjugate is designed with a long, flexible linker composed of PEG and ethylene-type segments (29-atom length) to preserve streptavidin’s biotin-binding activity. Each lot of conjugate consists primarily of over three streptavidin molecules labeled R-PE conjugates (high loading conjugates), purified by size-exclusion chromatography.
This product is sold as one vial of 100 microgram (Cat# CM54203-100UG) or 1 vial of 500 micrograms (Cat# CM54203-500UG). For bulk orders, please contact us for a quote.
Application
Suitable for detection of biotinylated antibodies and molecules in fluorescence-based assays, including flow cytometry, ELISAs, and microarrays.
Key Features
- Streptavidin highly loaded R-PE conjugates, purified by size-exclusion chromatography for high purity and performance.
- Retention of biotin binding
- Conjugation verified and quantified by UV and HPLC
Chemical Information
- Chemical name: R-PE-Streptavidin HL Conjugate
- Chemical formula: N/A
- Molecular weight: ≥400 KDa CAS number: N/A
Specification
- Physical appearance: Red-pink colored solution in 1 x PBS buffer without preservatives
- Storage temp.: Store at 2-8°C. Do not freeze.
- Purity: ≥ 99 % by SEC HPLC
- Number of Biotin Binding Sites per Conjugate: ≥10 (Refer to the COA for Lot-specific results)
- Excitation Max: 565 nm
- Emission Max: 576 nm
