Product Description
| Download Documents |
| Product Sheet |
CellMosaic’s thiol assay kit is designed to assay the free thiol group content of a biopolymer, such as a partially reduced antibody. CellMosaic routinely uses this kit for its internal bioconjugation-related research.
The assay is based on Ellman’s assay using 5’5’-dithio-bis-(2-nitrobenzoic acid) (DTNB) (Ellman, G.L. 1959, Tissue sulfhydryl groups. Arch Biochem Biophys. 82, 70–77). Thiol is reacted with DTNB to generate 2-nitro-5-thiobenzoic acid (TNB). TNB is orange in color and has an extinction coefficient of 14,150 M-1cm-1 at 412 nm (Riddles, P.W. Blakeley, R. L., and Zerner, B. 1983, Reassessment of Ellman’s reagent. Methods Enzymol. 91, 49-60). The amount of thiol groups will be calculated based on the amount of TNB generated.
Four micro-centrifuge tubes per package. Each package is sufficient for 10 assays (100 μL per assay volume).
Application of the product:
-
Assay the free thiol groups
Key Features of the product:
-
Less than 30 minutes of preparation and assay time. Fast and easy to use.
Frequently Asked Questions:
The list below includes FAQs that are specific to this product.
Question: What is the difference between CM90004 and CM90005?
Answer: CM90004 (thiol assay kit) is for checking purified biopolymer containing a thiol group. CM90005 (thiol assay kit with purification) is for assaying thiol groups after biopolymer modification. This kit includes a purification spin column to remove unreacted reducing reagents or thiolation reagents before the assay.
If you can’t find the answer you’re looking for or need information on general topics, please visit the main Frequently Asked Questions (FAQs) section.
