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    Main Menu

  • About Us
    • Our History
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    • Our Approach
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  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
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    • Custom Conjugation Guide
      • Small Molecule Supply
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    • ADC Controls
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    • Others
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  • Services
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  • Thiol assay kit CM90004 Ellman's assay
  • Thiol assay kit CM90004 Ellman's assay

    Thiol Assay Kit

    MSRP:
    Now: $127.00
    (You save )
    SKU:
    CM90004
    Availability:
    In stock, usually ships within 24 hrs
    Shipping:
    Calculated at Checkout
    Number of Assay:
    10

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    • Overview

    Product Description

     

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    Product Sheet

    Product Description

    CellMosaic’s thiol assay kit is designed to assay the free thiol group content of a biopolymer, such as a partially reduced antibody. CellMosaic routinely uses this kit for its internal bioconjugation-related research.
    The assay is based on Ellman’s assay using 5’5’-dithio-bis-(2-nitrobenzoic acid) (DTNB) (Ellman, G.L. 1959, Tissue sulfhydryl groups. Arch Biochem Biophys. 82, 70–77). Thiol is reacted with DTNB to generate 2-nitro-5-thiobenzoic acid (TNB). TNB is orange in color and has an extinction coefficient of 14,150 M-1cm-1 at 412 nm (Riddles, P.W. Blakeley, R. L., and Zerner, B. 1983, Reassessment of Ellman’s reagent. Methods Enzymol. 91, 49-60). The amount of thiol groups will be calculated based on the amount of TNB generated.

    Four micro-centrifuge tubes per package. Each package is sufficient for 10 assays (100 μL per assay volume).


    Application of the product:

    • Assay the free thiol groups

    Key Features of the product:

    • Less than 30 minutes of preparation and assay time. Fast and easy to use.

     

    Product Videos

    Custom Field

    Number of Assay 10

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