Product Description
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| SDS | USER MANUAL |
This kit is designed to label any IgG antibody with a VC-PAB linker. The final labeled product can be used as a negative control for any ADC with VC-PAB linker via thiol labeling chemistry. The preparation process is essentially the same as the standard ADC preparation with a VC-PAB linker. The user provides the antibody, and the kit includes maleimide-activated VC-PAB-OH, which can be coupled directly to the antibody following reduction and alkylation in a single step (a method developed by Seattle Genetics: Sun et al., 2005, Bioconjugate Chem. 16, 1282-1290). The kit also provides materials to purify the ADC control by removing any unreacted linkers.
This kit contains materials to conjugate 1-3 mg of one (CM11429x1) or three (CM11429x3) antibody samples (IgG).
If you have a smaller amount of IgG, a non-IgG type antibody, or other customization needs, please select the customization option and/or fill out the information above.
Linker Information
- Name: Mc-VC-PAB-OH
- CAS number: 159857-80-4
- Chemical formula: C28H40N6O7
- MW: 572.7 Da
Requirement for Antibody
- Preferably >90% pure by gel electrophoresis
- Total amount: 1-3 mg protein content as measured by UV
Key Features of this PerKit™
- Simple and efficient labeling of IgG with cathepsin B cleavable VC-PAB linker (Ref. Doronina et al. 2008, Bioconjugate Chem. 19, 1960-1963).
- Delivers an average of 4 Mc-VC-PAB-OH molecules per antibody.
- Fast preparation: 4 hours total, with less than 1 hour of hands-on time.
- Includes all necessary reagents and supplies for preparation and purification.
- Achieves over 95% conjugation, free from unreacted linker.
Conjugation Chemistry
Protocol
LAR: Linker-to-Antibody Ratio (LAR): check the user manual other consideration regarding the LAR calculation.
