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  • ADC Control Kit with VC-PAB Linker
  • ADC Control Kit with VC-PAB Linker
  • ADC Control Kit with VC-PAB Linker ADC Control Kit with VC-PAB Linker

    ADC Control Kit with VC-PAB Linker

    PerKit™

    MSRP:
    Now: $629.00
    (You save )
    SKU:
    CM11429
    Availability:
    In stock, usually ships within 1-3 days
    Shipping:
    Calculated at Checkout
    Reaction Scale:
    1-3 mg
    Linker Labeling Sites:
    Reduced thiols at heavy chains
    Linkage:
    Stable thiol ether bond

    Maximum file size is 200, file types are bmp, gif, jpg, jpeg, jpe, jif, jfif, jfi, png, wbmp, xbm, tiff, pdf

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    • Overview

    Product Description

     

    Download Documents
    SDS USER MANUAL

    This kit is designed to label any IgG antibody with VC-PAB linker. The final labeled product can be used as a negative control for any ADC with VC-PAB linker via thiol labeling chemistry. The preparation is essentially the same as standard ADC preparation with VC-PAB linker. The user supplies the antibody. The kit includes maleimide-activated VC-PAB-OH, which can be coupled directly to the antibody after reduction through alkylation in a single step (a method developed by Seattle Genetics: Sun et al. 2005, Bioconjugate Chem. 16, 1282-1290). The product is purified to remove any unreacted linkers.

    This kit provides materials to conjugate 1-3 mg of one (CM11429x1) or three (CM11429x3) antibody samples (IgG).

    If you have less amount of IgG, non-IgG type antibody, or other customization need, please select the customization radio button or fill out the information above.


    Linker Information

    • Name: Mc-VC-PAB-OH
    • CAS number: 159857-80-4
    • Chemical formula:  C28H40N6O7
    • MW: 572.7 Da

    Requirement for Antibody

    1. Preferably >90% pure by gel electrophoresis
    2. Total amount: 1-3 mg protein content measured by UV

    Key Features of this PerKit™

    • Offers a simple and easy way to prepare negative control ADC with cathepsin B cleavable VC-PAB linkage (Ref. Doronina et al. 2008, Bioconjugate Chem. 19, 1960-1963)
    • Stable linkage
    • Fast and easy preparation: 6 h preparation with <2 h hands-on time
    • All reagents and supplies included for preparation and purification
    • More than 95% labeled products (free of unreacted linker)
    • Options to choose tailored services at CellMosaic after conjugation

    After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and calculation of the loading.


    Conjugation Chemistry

    synthetic-route-cm11429.png


     Protocol

    scheme-cm11429.png


    Example of Preparing Control ADC

    Antibody information: A therapeutic antibody (human IgG1 subtype)

    Kit Lot number: S313.S9.022221

    Scale of the reaction: 1.42 mg of antibody (protein content)

    Figure 1: HIC HPLC analysis of IgG1 (blue trace) and purified IgG1 labeled with VC-PAB linker (red trace). 

    hic-analysis-of-example-control-adc-cm11429.png

     

    Figure 2. SEC HPLC analysis of IgG1 (blue trace) and purified IgG1 labeled with VC-PAB linker (red trace).

    sec-of-example-control-adc-cm11429.png

     

    Summary of the results:

    R value (SEC and HIC)

    0.75

    Average LAR based on R value

    3.2

    Average LAR based on HIC data

    3.0

    Extent of antibody aggregation (%)

    0

    Unreacted antibody (%)

    0

    Unreacted Mc-VC-PAB-OH (%)

    0

    Recovery (%)

    82

    LAR: Linker-to-Antibody Ratio (LAR): check the user manual other consideration regarding the LAR calculation.

     

    Product Videos

    Custom Field

    Reaction Scale 1-3 mg
    Linker Labeling Sites Reduced thiols at heavy chains
    Linkage Stable thiol ether bond

    Product Reviews

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