Product Description
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| Product Sheet | |
| SDS | COA_Lot#0531D |
MMAF (monomethyl auristatin F) is a new antimitotic auristatin derivative with a charged C-terminal phenylalanine residue. Mc-VC-PAB-MMAF is a thiol reactive derivative of MMAF that contains a cathepsin B cleavable valine-citruline p-aminobenzylcarbamate linkage (VC-PAB). This product is a control ADC for any IgG type antibody labeled with MMAF, particularly any ADC made using CellMosaic's Antibody MMAF Conjugation Kit (Cat#: CM11425 and Cat#: CM11422). The product uses a human IgG1 subtype antibody that does not bind to the target. It has an average of 3-5 MMAF loaded on each antibody and can be used for assessing the effect of MMAF alone (non-specific toxicity). The product uses a very low endotoxin level antibody as a starting material (≤0.5 EU/mg by the LAL method). The preparation and purification are conducted under a pyrogen free environment.
The final conjugate is lyophilized from pH 6.5 citrate buffer containing sugar-based stabilizer for easy shipping and storage.
The product is sold as 1 vial of 100 microgram (Cat# CM51101-100UG), 1 vial of 500 micrograms (Cat# CM51101-500UG), or 2 vials of 500 micrograms (Cat# CM51101-1MG). For bulk orders, please contact us for a quote.
Chemical Information
- Chemical name: ADC Control hIgG1-MMAF
- Chemical formula: N/A
- Molecular weight: 152KDa CAS number: N/A
Specification
- Physical Appearance: Colorless to white lyophilized powder in a microcentrifuge tube
- Storage Temp.: -20°C
- Purity: ≥99% of MMAF labeled antibody by SEC HPLC, free of any unreacted MMAF
- Endotoxin Level: ≤0.5 EU/mg by the LAL method
- Drug Over Antibody Ratio (DAR): 3 to 5 by UV ratio, HIC HPLC, and difference MALDI-TOF MS
Antibody Information
- Immunogen: HEL (Hen Egg Lysozyme)
- Species Reactivity: chicken
- Expression Host: CHO-S cell line
- Class: Human IgG1, Kappa
- Antibody Type: Monoclonal
- Applications: ELISA, FACS, IF, IHC, in vivo studies, IP, WB
- Purification Method: Affinity purified
Drug Information
- Name: Monomethyl auristatin F (MMAF) with Mc-VC-PAB linker
- CAS number: N/A
- Mechanism of action: Inhibits cell division by blocking the polymerization of tubulin. VC-PAB linker is stable in extracellular fluid but cleaved by cathepsin B once inside the tumor cell, activating the antimitotic mechanism.
- Activities: Antioxidant, anti-inflammatory, anticancer, and insecticidal activities.
- Selected References: 1) Doronina SO, Mendelsohn BA, Bovee TD, et al. Enhanced activity of monomethylauristatin F through monoclonal antibody delivery: effects of linker technology on efficacy and toxicity. Bioconjug Chem 2006;17: 114–24. 2) Polson AG, et al. Antibody-drug conjugates for the treatment of non-Hodgkin's lymphoma: target and linker-drug selection. Cancer Res. 2009;69(6):2358−2364.
Related Products
Antibody MMAF Conjugation Kit (With VC-PAB Linkage) (Product #: CM11425): ADC kit designed for labeling any IgG type antibody with MMAF using a cleavable VC-PAB linker for early stage screening of customer's antibody.
Antibody Mc-MMAF Conjugation Kit (Product #: CM11422): ADC kit designed for labeling any IgG type antibody with MMAF with stable thiol ether bond for early stage screening of customer's antibody.
