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APPLICATION NOTE |
CellMosaic ADC PerKits™ are available to prepare antibody drug conjugates through surface amines or reduced thiols. Some ADC kits apply to general payloads with certain functional groups. Some ADC kits supply the selected, standard linkage molecules conjugated to a selected payload, ready with the appropriate reactive modifications to directly attach the payload and linker to your specific antibody of interest. The kits are complete with all reagents and procedures for attaching the selected payload directly to your antibody and purifying the final ADC. All you need to supply is your antibody and standard lab equipment.
A typical CellMosaic personalized ADC kit results in the preparation of approximately 2-3 mg of final ADC in buffer with an average DAR of 2-4. Purities are typically >90% conjugate, free of or with less than 5% of unreacted payload. Individual results can vary depending on the specific antibody system you are using and the hydrophobicity of your payload. Kit instructions are complete, easy to follow, and can typically be carried out within 6 hours (with less than 1-hour hands-on time).
Other products on the market for antibody screening may utilize a general secondary antibody, or a general antibody affinity coupling (such as protein-A), to provide indirect conjugation, but these indirect conjugation approaches for ADCs can significantly impact the hydrodynamic size and physiological behaviors (for example, changing cleavage and degradation mechanisms), so these products do not provide a fully representative approach for evaluating your novel ADC in screening or for proof-of-concept (POC) studies.
Product# |
Protein |
Drug |
Scale of Reaction (Standard Kit) |
Labeling Chemistry |
Linkage |
CM11429 | IgG Ab | No drug (VC-PAB linker only) | 1-3 mg | Reduced thiol | Releasable |
CM51403 | IgG Ab |
Acid |
1-3 mg | Surface amine | Stable |
CM11410 (x1, x3) | IgG Ab | DM1 | 0.1 mg, 1-3 mg | Surface amine | Stable |
CM11406 (x1, x3) | IgG Ab | Doxorubicin | 1-3 mg | Surface amine | Stable |
CM11407 (x1, x3) | IgG Ab | Methotrexate | 1-3 mg | Surface amine | Stable |
CM11409 (x1, x3) | IgG Ab | MMAE | 0.1 mg, 1-3 mg, 30 mg | Reduced thiol | Releasable |
CM11422 (x1, x3) | IgG Ab | MMAF | 1-3 mg | Reduced thiol | Stable |
CM11425 (x1, x3) | IgG Ab | MMAF | 1-3 mg | Reduced thiol | Releasable |
CM11408 (x1, x3) | IgG Ab | SN38 | 1-3 mg, 30 mg | Surface amine | Releasable |
CM11431 (x1, x3) | IgG Ab | Deruxtecan | 1-3 mg | Reduced thiol | Releasable |
CM11416 (x1, x3) | F(ab')2 | MMAE | 0.73-2.2 mg | Reduced thiol | Releasable |
F(ab')2 | DM1 | 0.73-2.2 mg | Surface amine | Stable |
AqT™ ADC Drug Development & ManufacturingIn the event that a drug conjugate cannot be made using classical linkers or the effectiveness of the drug conjugates are compromised by the number of toxins loaded onto the antibody using CellMosaic PerKits™ ADC, CellMosaic has developed super-hydrophilic and water soluble AqT™ linker for this purpose. Click here for more information. |
1. Lofgren, Kristopher A.; Sreekumar, Sreeja; Jenkins, Charles E., Jr.; Ernzen, Kyle J.; Kenny, Paraic A. "Anti-tumor efficacy of an MMAE-conjugated antibody targeting cell surface TACE/ADAM17-cleaved Amphiregulin in breast cancer." Antibody Therapeutics 2021, 4 (4), 252-261.
2. Neetha Parameswaran, Liping Luo, Lingjun Zhang, Joel Chen, Frank P. DiFilippo, Charlie Androjna, David A. Fox, Sarah L. Ondrejka, Eric D. Hsi, Deepa Jagadeesh, Daniel J. Lindner & Feng Lin. "CD6-targeted antibody-drug conjugate as a new therapeutic agent for T cell lymphoma" Leukemia 2023, 37, 2050-2057.
3. Micalizzi, Douglas S.; Che, Dante: Nicholson, Benjamin T.; Edd, Jon F.; Desai, Niyati; Lang, Evan R.; Toner, Mehmet; Maheswaran, Shyamala; Ting, David T.; Haber, Daniel A. "Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody." PNAS 2022, 43 (119), e2209563119.