Product Description
AqT® Biotin Goat Anti-Human IgG is a novel biotinylated polyclonal secondary antibody developed with CellMosaic's proprietary AqueaTether® (AqT®) linker technology, delivering enhanced assay performance.
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AqT® Biotin Goat Anti-Human IgG is a biotinylated polyclonal secondary antibody specific for the F(ab′)₂ region of human IgG. The antibody is cross-adsorbed to minimize cross-reactivity with bovine, horse, and mouse serum proteins.
The product is site-specifically biotinylated at reduced Fc-region disulfide bonds using CellMosaic’s proprietary super-hydrophilic AqT® linker. By positioning biotin away from the antigen-binding regions, this controlled labeling approach is designed to help preserve antibody-binding activity while providing consistent and accessible biotin placement.
Each antibody contains an average of 3–5 biotin molecules, supporting strong streptavidin-mediated signal amplification and sensitive, reproducible detection across a variety of immunoassay applications.
For more information about AqT® Biotinylated Secondary Antibodies, visit:
https://cellmosaic.com/biotinylated-secondary-antibodies/
This product is sold as 1 tube of 100 µg conjugate (CM86152-100UG). For bulk quantities and custom packaging requirements, please contact us for pricing and availability.
For a detailed description of the AqT® technologies, please see our technology section.
Key Features
- Site-specific biotinylation with an average of 3–5 biotin molecules per antibody.
- Protein content determined by UV/HPLC analysis.
- Biotin incorporation measured using a biotin assay.
- Cross-adsorbed to minimize cross-reactivity with bovine, horse, and mouse serum proteins
- Advanced AqT® linker technology: Utilizes CellMosaic’s proprietary, super-hydrophilic, water-soluble, charge-neutral AqT® linker to enhance aqueous solubility and minimize aggregation.
Applications
- Enzyme-linked immunosorbent assays (ELISA)
- Flow Cytometry, Immunohistochemistry (IHC)
- Immunocytochemistry (ICC)
- Western Blotting
- Immunoprecipitation (IP) and Pull-Down Assays
- Biosensor and Surface Immobilization, including SPR and BLI
- Cell Isolation and Magnetic Separation
Specifications
- Physical Appearance: Clear solution in 1xPBS buffer with stabilizers.
- Storage Temp: –20°C or below.
- Purity: ≥95% of monomeric by SEC-HPLC (refer COA of each lot for actual value).
- Amount of Biotin Loaded per BSA Molecule: 3–5 (refer COA of each lot for actual value).
Antibody Information
- Target: Human IgG
- Immunogen: Human IgG F(ab')₂ fragment
- Species Reactivity: Human
- Cross-Adsorption: Bovine, horse, and mouse serum proteins
- Host Species: Goat
- Class/Isotype: Goat IgG
- Antibody Type: Polyclonal
- Country of Manufacture: USA
Characterization of AqT® Goat Anti-Human IgG, F(ab')2 Specific, Cross-Adsorbed by HPLC
Lot Number: S359.S9.0811F (Biotin Loading: 4.1 by Biotin Assay)
Figure 1. Overlay SEC-HPLC profiles of F(ab′)₂-specific goat anti-human IgG before labeling (blue trace; ~3% aggregation, indicated by the peak at 9.977 minutes) and after AqT® biotin labeling (red trace). The biotinylated antibody (Lot No. S359.S9.0811F; DOL: 4.1) demonstrated ≥99% monomeric purity in PBS, with no detectable aggregate peak.
Note: SEC separates the conjugates by apparent MW or size in aqueous solution. In general, higher-MW species elute earlier. SEC is also a useful tool for assessing the level of aggregation in a biopolymer. By comparing the SEC profiles of an unlabeled protein and labeled protein, you can estimate the extent of aggregation in the labeled protein.
Frequently Asked Questions:
If you can’t find the answer you’re looking for or need information on general topics, please visit the main Frequently Asked Questions (FAQs) section.
Other Details
The information in this document is subject to change without notice. CellMosaic® assumes no responsibility for any errors or omissions that may appear in this document. Under no circumstances shall CellMosaic be liable, whether in contract, tort, warranty, or under any statute or on other legal theory, for special, incidental, indirect, punitive, multiple, or consequential damages related to or arising from this document, including but not limited to the use of this product thereof.CellMosaic’s products, including but not limited to AqT®, NeIon™, oxLink™ and sxLink™ (collectively, the “Products”), are covered by, or are the subject of, one or more issued patents and pending patent applications, including without limitation: 9,688,663 B2; 8,907,079 B2; 9,511,150 B2; 9,907,854 B2; 9,346,833 B2; 9,518,067 B2; CN104066451B; CN107043339B; CA 2,841,313; EP 2734238 B1; AU2012284055; JP 6240599 B2; and corresponding foreign patents and patent applications. The purchase of the Product conveys solely a limited, non-exclusive, non-transferable, non-sublicensable license to use the Product only for the purchaser’s internal research and development purposes. No right or license is granted, either expressly or by implication, estoppel, or otherwise, under any patent, trademark, copyright, or other intellectual property right of CellMosaic, except as expressly stated herein. Any commercial use of the Product is strictly prohibited without a separate written agreement from CellMosaic. Prohibited commercial uses include, without limitation: (a) the sale, lease, licensing, distribution, or other transfer of the Product or any materials derived from or produced using the Product; (b) the sale, lease, licensing, or other grant of rights to use the Product or any materials derived from or produced using the Product; and (c) the use of the Product to perform services for a fee or other consideration for third parties, including without limitation contract research, screening services, or diagnostic or therapeutic applications. For information regarding commercial licensing or collaboration, please contact us at info@cellmosaic.com.
